Induction of meiotic gynogenesis in paddlefish (Polyodon spathula) and its confirmation using microsatellite markers
文献类型: 外文期刊
作者: Zou, Y. C. 1 ; Wei, Q. W. 2 ; Pan, G. B. 2 ;
作者机构: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
2.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agrculture, Yangtze River Fisheries Res Inst, Jinzhou, Hubei, Peoples R China
3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China
关键词: microsatellite marker;UV irradiation;maternal inheritance;meiotic gynogenesis;diploid gynogenesis;gynogenetic progeny;gynogenesis induction;haploid control
期刊名称:JOURNAL OF APPLIED ICHTHYOLOGY ( 影响因子:0.892; 五年影响因子:0.956 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A protocol for gynogenesis of P. spathula was developed in order to produce inbred lines and to develop a technique to preserve the endangered Chinese paddlefish (Psephurus gladius). Diploid gynogenesis was induced in P. spathula using ultraviolet (UV)-irradiated Amur sturgeon (Acipenser schrenckii) sperm for 4.5-min, 5-min or 5.5-min and subsequent heat shock treatment. UV irradiation of sperm for 5 min at a UV intensity (254 nm) of 863 mu w cm(-2) was the optimum dose to achieve diploid gynogenesis on the basis of observations on hatching rate of eggs. Three microsatellite loci were used to monitor exclusive maternal inheritance of gynogenetic progenies. The results showed all maternal genome among offspring with no paternal genome. The cytogenetic analysis showed that meiotic gynogenetic diploids possessed 120 chromosomes in metaphase plates, while haploid control groups N1-3 possessed 60 chromosomes.
- 相关文献
作者其他论文 更多>>
-
Inactivated Mycobacterium vaccine induces innate immunity against M. ulcerans ecovar Liflandii (MuLiflandii ASM001) infection in Dabry's sturgeon (Acipenser dabryanus)
作者:Huang, J.;Wei, Q. W.;Huang, J.;Zhang, S. H.;Di, J.;Du, H.;Wei, Q. W.;Huang, J.
关键词:
-
Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation
作者:Xi, M. D.;Wei, Q. W.;Xi, M. D.;Wei, Q. W.;Xi, M. D.;Li, P.;Du, H.;Qiao, X. M.;Liu, Z. G.;Wei, Q. W.;Xi, M. D.;Li, P.;Du, H.;Qiao, X. M.;Liu, Z. G.;Wei, Q. W.;Li, P.
关键词:cryopreservation; disaccharides; enolase3; plasma membrane Ca2+-ATPase isoform; sperm
-
Antioxidant supplementation, effect on post-thaw spermatozoan function in three sturgeon species
作者:Li, P.;Xi, M. D.;Du, H.;Qiao, X. M.;Liu, Z. G.;Wei, Q. W.;Li, P.;Xi, M. D.;Du, H.;Qiao, X. M.;Liu, Z. G.;Wei, Q. W.;Li, P.
关键词:antioxidant; cryopreservation; sperm quality; spermatozoa; sturgeon
-
ECOLOGICAL EFFECTS OF THE FIRST DAM ON YANGTZE MAIN STREAM AND FUTURE CONSERVATION RECOMMENDATIONS: A REVIEW OF THE PAST 60 YEARS
作者:Zhang, H.;Li, J. Y.;Wu, J. M.;Wang, C. Y.;Du, H.;Wei, Q. W.;Kang, M.
关键词:ecological impact assessment; anadromous fish; movement and migration; Gezhouba Dam; Yangtze River
-
PHYSICAL HABITAT ASSESSMENT OF A REMAINING HIGH-BIODIVERSITY REACH OF THE UPPER YANGTZE RIVER, CHINA
作者:Zhang, H.;Wang, C. Y.;Wu, J. M.;Du, H.;Wei, Q. W.;Kang, M.
关键词:river habitat survey;echosounder;ADCP;river management;protected area
-
Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus
作者:Cheng, W. W.;Wang, D. Q.;Wang, C. Y.;Du, H.;Wei, Q. W.;Cheng, W. W.
关键词:Chinese sucker;Microsatellite;Genetic differentiation;Population structure;Conservation
-
RIVER TEMPERATURE VARIATIONS AND POTENTIAL EFFECTS ON FISH IN A TYPICAL YANGTZE RIVER REACH: IMPLICATIONS FOR MANAGEMENT
作者:Zhang, H.;Wu, J. M.;Wang, C. Y.;Du, H.;Liu, Z. G.;Shen, L.;Wei, Q. W.;Chen, D.
关键词:river temperature regime;natural reproduction;Acipenser sinensis;four major Chinese carps;Yichang Hydrological Monitoring Station