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Induction of meiotic gynogenesis in paddlefish (Polyodon spathula) and its confirmation using microsatellite markers

文献类型: 外文期刊

作者: Zou, Y. C. 1 ; Wei, Q. W. 2 ; Pan, G. B. 2 ;

作者机构: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China

2.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agrculture, Yangtze River Fisheries Res Inst, Jinzhou, Hubei, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China

关键词: microsatellite marker;UV irradiation;maternal inheritance;meiotic gynogenesis;diploid gynogenesis;gynogenetic progeny;gynogenesis induction;haploid control

期刊名称:JOURNAL OF APPLIED ICHTHYOLOGY ( 影响因子:0.892; 五年影响因子:0.956 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A protocol for gynogenesis of P. spathula was developed in order to produce inbred lines and to develop a technique to preserve the endangered Chinese paddlefish (Psephurus gladius). Diploid gynogenesis was induced in P. spathula using ultraviolet (UV)-irradiated Amur sturgeon (Acipenser schrenckii) sperm for 4.5-min, 5-min or 5.5-min and subsequent heat shock treatment. UV irradiation of sperm for 5 min at a UV intensity (254 nm) of 863 mu w cm(-2) was the optimum dose to achieve diploid gynogenesis on the basis of observations on hatching rate of eggs. Three microsatellite loci were used to monitor exclusive maternal inheritance of gynogenetic progenies. The results showed all maternal genome among offspring with no paternal genome. The cytogenetic analysis showed that meiotic gynogenetic diploids possessed 120 chromosomes in metaphase plates, while haploid control groups N1-3 possessed 60 chromosomes.

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