Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages
文献类型： 外文期刊
作者： Cao, H. 1 ; Zhou, R. J. 2 ; Wei, Q. W. 3 ; Li, C. J. 3 ; Gui, J. F. 1 ;
作者机构： 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Agr Minist China, Yangtze River Fisheries Res Inst, Jinzhou, Peoples R China
关键词： growth hormone: 9002-72-6;complementary DNA: cDNA;polyclonal antibody;signal peptide;embryogenesis
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
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收录情况： SCI
摘要： Chinese sturgeon (Acipenser sinensis Gray) has a fast growth rate starting in early larval stage. Likely, the fast growth is attributed to the growth hormone (GH); an essential polypeptide required for growth and development of vertebrates. In this study, GH cDNA was cloned from a pituitary library. The AsGH cDNA consists approximately 954 bp in size including a 16 bp 5'-untranslated region and 296 bp 3'-untranslated region. The open reading frame (642 bp) encodes a 214 amino acids (aa), which represents the precursor composed of a 25 aa signal peptide followed by a 189 aa mature polypeptide. The polyclonal antibody was prepared from the in vitro expressed mature protein. Western blot analysis showed a constant GH expression of a monomeric form in the 13 embryo stages and in several larval stages. Interestingly, two dimeric bands of GH were detected since day 3 in the hatched larvae. Our findings suggest that the GH may play important physiological functions in embryonic and larval development of Chinese sturgeon.
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