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Low-level expression of cholesterol 7 alpha-hydroxylase is associated with the formation of goose fatty liver

文献类型: 外文期刊

作者: Zhao, A. Y. 1 ; Wang, X. D. 2 ; Chen, G. H. 1 ; Lu, L. Z. 3 ;

作者机构: 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China

2.Zhejiang Agr & Forestry Univ, Dept Anim Sci, Hangzhou 311300, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet, Hangzhou 310021, Zhejiang, Peoples R China

关键词: triglyceride;low density lipoprotein: LDL;cholic acid: 81-25-4;synthesis;cholesterol: 57-88-5;catabolism;cholesterol 7-alpha-hydroxylase: 9037-53-0;expression;EC 1.14.13.17

期刊名称:POULTRY SCIENCE ( 影响因子:3.352; 五年影响因子:3.679 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Cholesterol 7 alpha-hydroxylase (Cyp7a1) plays a crucial role in the synthesis of cholic acid and cholesterol catabolism. In this study, Cyp7a1 expression in goose liver was identified for the first time using differential display reverse transcription PCR. This study used real-time PCR and quantified the transcript levels of the cyp7a1 gene under different feeding conditions. In overfeeding, cyp7a1 expression by the liver was lower than that seen for normal feeding and the same result was observed for the Cyp7a1 protein level. The cholesterol level in serum and liver was higher in the overfed group. This study suggests that low-level expression of Cyp7a1 is associated with the formation of goose fatty liver.

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