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Cloning and characterization of a hsp70 gene from Asiatic hard clam Meretrix meretrix which is involved in the immune response against bacterial infection

文献类型： 外文期刊

作者： Yue, Xin 1 ; Liu, Baozhong 1 ; Sun, Li 1 ; Tang, Baojun 3 ;

作者机构： 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

3.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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收录情况： SCI

摘要： In the present study, a 71.43kDa heat shock protein cDNA was cloned from Asiatic hard clam Meretrix meretrix. The cDNA was 2292bp, containing an open reading frame (ORF) of 1959bp, which encodes a protein of 652 amino acids with a theoretical molecular weight of 71.43kDa and an isoelectric point of 5.32. Based on the amino acid sequence analysis and phylogenetic analysis, this hsp70 cDNA is a member of cytoplasmic hsc70 (constitutive genes) subfamily in the hsp70 family, and is designated as MmeHsc71. Quantitative RT-PCR was carried out to compare the spatial and temporal expression patterns of MmeHsc71 in the mRNA level between control clams and Vibrio parahaemolyticus-infected clams. Spatially, MmeHsc71 mRNA was found in all tested tissues, including foot, hepatopancreas, mantle and gill. MmeHsc71 mRNA expression level in hepatopancreas and gill displayed a significant increase in vibrio-challenged clams at 24h post-infection compared to control clams (P <0.05). Temporally, there was a significant increase of MmeHsc71 mRNA level in hepathopancreas of vibrio-challenged clams compared to control clams at 6, 12, and 24h post-challenge, respectively. The result of quantitative immunofluorescence also indicated that there was obvious increase of MmeHsc71 in hepatopancreas of vibrio-challenged clams compared to control clams in protein level at 24h post-infection. The results suggested that MmeHsc71 may play an important role in mediating the immune responses of M. meretrix to bacterial challenge. a-[ordm A 71.43 kDa heat shock protein was cloned from clam Meretrix meretrix. a-[ordm The heat shock protein obtained is a member of hsc70 subfamily and was designated as MmeHsc71. a-[ordm Quantitative immunofluorescence and RT-PCR examination were used to detect the expression changes of MmeHsc71 in response to vibrio-challenge. a-[ordm The elevated mRNA and protein levels of MmeHsc71 after vibrio-challenge suggested the immune-related function of MmeHsc71 in M. meretrix.

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