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Characterization and antioxidant activities of polysaccharides from Hippophae rhamnoides L

文献类型： 外文期刊

作者： Bao, Xiaowei 1 ; Wang, Wei 1 ; Song, Suqing 2 ; Wei, Chenye 1 ; Li, Yixin 1 ; Sun, Jiali 1 ; Jiang, Junfeng 1 ;

作者机构： 1.Xinjiang Agr Univ, Coll Food Sci & Pharm, Urumqi 830052, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Xinjiang Key Lab Special Environm Microbiol, Urumqi 830091, Peoples R China

关键词： Hippophae rhamnoides L. polysaccharide characterization antioxidant activity

期刊名称：JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION （ 影响因子：3.4； 五年影响因子：3.2 ）

ISSN： 2193-4126

年卷期： 2023 年

页码：

收录情况： SCI

摘要： In this paper, the optimal extraction conditions of crude polysaccharide from Hippophae rhamnoides L. were gained by response surface methodology (RSM) according to the principle of Box-Behnken design (BBD), and three purified fractions (PBP-1, PBP-2 and PBP-3) were isolated by multiple column chromatography. PBP-1 mainly contained arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.18: 1: 2.20: 32.17: 1.45. PBP-2 mainly contained xylose, mannose, glucose and galactose in a molar ratio of 1: 0.28: 1.02: 0.2. PBP-3 mainly contained xylose, glucose and galactose in a molar ratio of 1: 2.15: 0.28, and the average molecular weights were 26.5, 5.6 and 4.9 kDa, respectively. Furthermore, the CPBP and PBP-3 exhibited the higher effective scavenging activities against DPPH, ABTS and reducing power, and the CPBP indicated the highest protective effect against H2O2-induced oxidative injury in LO2 cells. These findings could provide useful information for the utilization and development of the resources of Hippophae rhamnoides L.

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