北京市农林科学院机构知识库

Development of a simple molecular marker specific for detecting the self-compatible S4' haplotype in sweet cherry (Prunus avium L.)

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文献类型：外文期刊

作者： Zhu, M ¹ ; Zhang, XM ² ; Zhang, KC ² ; Jiang, LJ ² ; Zhang, LM ² ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China; Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China; China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China

关键词： haplotype;prunus avium L.;S haplotype-specific F-box protein gene;self-incompatibility;PEAR PYRUS-SEROTINA;BOX PROTEIN GENE;INCOMPATIBILITY ALLELES;PCR DETECTION;IDENTIFICATION;CLONING;RNASES;LOCUS;CDNA;MUME

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （影响因子：1.595；五年影响因子：2.042 ）

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收录情况： SCI

摘要： In sweet cherry (Prunus avium L.), the S4' haplotype, characterized by a self-incompatibility (SI) defect in pollen, is self-compatible and is derived from the self-incompatible S4 haplotype by x-ray mutagenesis. SFBs (S haplotype-specific F-box protein genes) have been found to associate with pollen determinant of SI. This report identified the SFB4' of the self-compatible S4' haplotype. The alignment of the sequences of SFB4' and SFB4 by the BLAST program revealed a 4-bp deletion in SFB4', which is TTTA. The sequence polymorphism generated by the TTTA deletion in SFB4' was exploited to develop a simple molecular marker specific for detecting the S4' but not the S4 haplotype. The simple marker specific to the S4' haplotype can be visualized directly on an agarose gel, so it can be immediately applied to a marker-assistant cherry-breeding program. Thus, this work provides a practical molecular marker for cherry breeding.

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Development of a simple molecular marker specific for detecting the self-compatible S4' haplotype in sweet cherry (Prunus avium L.)

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