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Cloning, characterization and expression of the gene encoding polygalacturonase-inhibiting proteins (PGIPs) of peach [prunus persica (L.) Batch]

文献类型: 外文期刊

作者: Liang, FS 1 ; Zhang, KC 2 ; Zhou, CJ 2 ; Kong, FN 2 ; Li, J 2 ; Wang, B 2 ;

作者机构: 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; Beijing Acad Agr & Forestry Sci, Forestry & Pomol Res Inst, Beijing 100093, Peoples R China; Hebei Agr Univ, Coll Agr, Baoding 071001, Peoples R China

关键词: polygalacturonase-inhibiting proteins (PGIPs);leucine-rich repeat (LRR);peach [prunus persica (L.) Batch];SIGNAL-TRANSDUCTION PATHWAYS;SALICYLIC-ACID;PHASEOLUS-VULGARIS;BOTRYTIS-CINEREA;DEFENSE;ARABIDOPSIS;PROMOTER;FRUITS;PLANTS

期刊名称:PLANT SCIENCE ( 影响因子:4.729; 五年影响因子:5.132 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Polygalacturonase-inhibiting Proteins (PGIPs) are plant proteins that counteract fungal Polygalacturonases (PGs), which are important virulence factors. As defense proteins, PGIP can efficiently limit fungal invasion. In this study, a full-length cDNA of PPGIPI gene (peach PGIP gene copy no. 1) has been cloned from peach [prunus persica (L.) Batch] variety 'Okubo', which is resistant to fungal diseases such as peach scab. Sequence analysis revealed that the cDNA of PPGIP] is 1380 bp in length and that its containing open reading frame (ORF) encodes for a polypeptide of 330 amino acids. The PPGIPI gene from genomic DNA shows a single 147 bp intron that is efficiently spliced out of the PPGIP1 pre-mRNA transcript. The PPGIP] has a high degree of identify with previously isolated PGIP genes and the encoded polypeptide shows all the characteristic features of PGIP peptides. Analysis of genomic organization and expression indicate that the PPGIP I gene is one of a low copy number gene family with three copies in peach genome and its expression can be induced by exogenous salicylic acid (SA). (C) 2004 Elsevier Ireland Ltd. All rights reserved.

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