Development of a reverse-transcription loop-mediated isothermal amplification assay for the detection of Tobacco mild green mosaic virus (TMGMV)
文献类型： 外文期刊
作者： Zhao, Wenhao 1 ; Wu, Shuhua 1 ; Du, Linlin 1 ; Li, Tingfang 1 ; Cheng, Zhaobang 1 ; Zhou, Yijun 1 ; Ji, Yinghua 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing 210014, Peoples R China
关键词： Tobacco mild green mosaic virus (TMGMV); Reverse-transcription loop-mediated; isothermal amplification (RT-LAMP); Virus detection; Pepper; Capsicum annuum
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2021 年 298 卷
页码：
收录情况： SCI
摘要： Tobacco mild green mosaic virus (TMGMV), a member species of the genus Tobamovirus, infects pepper (Capsicum annuum) and a number of other economically important species in the Solanaceae family. TMGMV infections had seriously impacted pepper production worldwide, including China. A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect TMGMV in pepper field samples and seed. This assay was based on four primers that matched to six sequences in the C-terminal region of the TMGMV genome. RT-LAMP assay could detect the presence of the virus in 3.0 x 10- 7 mu g of total RNA extract from pepper leaves, which was ten times more sensitive than the corresponding reverse-transcription polymerase chain reaction (RT-PCR) assay. This method specifically detected TMGMV but not the closely related species of the same genus Pepper mild mottle virus, Cucumber green mottle mosaic virus and Tomato mosaic virus. In addition, the use of SYBR Green I facilitated the detection of the TMGMV RT-LAMP products by the naked eye. These results indicated that the RT-LAMP assay was a simple, sensitive, specific and affordable diagnostic tool that has the potential to detect and monitor TMGMV infection in field samples.
- 相关文献
作者其他论文 更多>>
-
MicroRNA750-3p Targets Processing of Precursor 7 to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector Laodelphax striatellus
作者：Wang, Haitao;Dong, Yan;Xu, Qiufang;Wang, Man;Li, Shuo;Ji, Yinghua
关键词：microRNA750-3p; processing of precursor 7; rice black-streaked dwarf virus; small brown planthopper; Laodelphax striatellus; viral propagation
-
A plant NLR receptor employs ABA central regulator PP2C-SnRK2 to activate antiviral immunity
作者：Huang, Shen;Wang, Chunli;Ding, Zixuan;Zhao, Yaqian;Dai, Jing;Li, Jia;Huang, Haining;Wang, Tongkai;Zhu, Min;Feng, Mingfeng;Tao, Xiaorong;Ji, Yinghua;Zhang, Zhongkai
关键词：
-
Investigation and characterization of rice dwarfing epidemic caused by southern rice black-streaked dwarf virus in Jiangsu in 2023
作者：Li, Chenyang;Yang, Wenxuan;Du, Linlin;Lin, Feng;Lan, Ying;Zhou, Tong;Yang, Wenxuan;Zhang, Yuanming;Qiu, Yanglin;Xu, Kai;Zhou, Tong;Zhu, Feng
关键词：Rice viral disease; SRBSDV; Jiangsu rice dwarfing epidemic; Virus detection; Koch's postulates
-
Development of a transgenic rice line with strong and broad resistance against four devastating rice viruses through expressing a single hairpin RNA construct
作者：Li, Chenyang;Wu, Jianxiang;Fu, Shuai;Xu, Yi;Wang, Yaqin;Zhou, Xueping;Li, Chenyang;Lan, Ying;Lin, Feng;Du, Linlin;Zhou, Tong;Yang, Xiuling;Zhou, Xueping
关键词：rice; resistance; virus; RNA interference; hairpin RNA transgene
-
The miR-184-3p promotes rice black-streaked dwarf virus infection by suppressing Ken in Laodelphax striatellus (Fallén)
作者：Wu, Wei;Wang, Man;Deng, Zhiting;Xi, Minmin;Dong, Yan;Wang, Haitao;Zhang, Jianhua;Zhou, Yijun;Xu, Qiufang;Wu, Wei;Deng, Zhiting;Xi, Minmin;Wang, Changchun;Xu, Qiufang
关键词：miR-184-3p; Laodelphax striatellus; rice black-streaked dwarf virus; Ken; virus infection
-
Molecular Hydrogen Confers Resistance to Rice Stripe Virus
作者：Shao, Yudong;Lin, Feng;Wang, Zhaoyun;Liu, Zhiyang;Chen, Dongyue;Guo, Wei;Lan, Ying;Du, Linlin;Zhou, Yijun;Zhou, Tong;Shao, Yudong;Wang, Yueqiao;Cheng, Pengfei;Lou, Wang;Chen, Dongyue;Shen, Wenbiao
关键词：Arabidopsis; disease resistance; molecular hydrogen; rice; rice stripe virus; salicylic acid
-
Development of novel specific molecular markers for the Sw-5b gene to assist with tomato spotted wilt virus-resistant tomato breeding
作者：Tong, Cong;Huang, Shen;Shi, Yajie;Wu, Qian;Shangguan, Lingna;Yu, Haohua;Chen, Rongzhen;Ding, Zixuan;Xiao, Yunxia;Zhu, Min;Tao, Xiaorong;Ji, Yinghua;Zhang, Zhongkai;Zhang, Hui
关键词：Sw-5b; TSWV; Gene-specific molecular marker; Marker-assisted selection; Breeding; Tomato
