Cellulase with Bacillus velezensis improves physicochemical characteristics, microbiota and metabolites of corn germ meal during two-stage co-fermentation
文献类型: 外文期刊
作者: Chen, Long 1 ; Guo, Yang 1 ; Liu, Xin 1 ; Zheng, Lin 1 ; Wei, Bingdong 1 ; Zhao, Zijian 2 ;
作者机构: 1.Jilin Acad Agr Sci, Inst Anim Nutr & Feed, 186 Dong Xinghua St, Gongzhuling 136100, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Inst Agro Food Technol, 1366 Cai Yu St, Changchun 130033, Jilin, Peoples R China
关键词: Nutritional value; Two-stage solid-state fermentation; Microbial diversity; Fermented feed
期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:4.1; 五年影响因子:4.2 )
ISSN: 0959-3993
年卷期: 2024 年 40 卷 2 期
页码:
收录情况: SCI
摘要:
Corn germ meal (CGM) is one of the major byproducts of corn starch extraction. Although CGM has rich fiber content, it lacks good protein content and amino acid balance, and therefore cannot be fully utilized as animal feed. In this study, we investigated the processing effect of cellulase synergized with Bacillus velezensis on the nutritional value of pretreated CGM (PCGM) in two-stage solid-state fermentation (SSF). High-throughput sequencing technology was used to explore the dynamic changes in microbial diversity. The results showed that compared with four combinations of B. velezensis + Lactiplantibacillus plantarum (PCGM-BL), cellulase + L. plantarum (PCGM-CL),control group (PCGM-CK), and cellulase + B. velezensis + L. plantarum (PCGM-BCL), the fourth combination of PCGM-BCL significantly improved the nutritional characteristics of PCGM. After two-stage SSF (48 h), viable bacterial count and contents of crude protein (CP) and trichloroacetic acid-soluble protein (TCA-SP) all were increased in PCGM-BCL (p < 0.05), while the pH was reduced to 4.38 +/- 0.02. In addition, compared with PCGM-BL, the cellulose degradation rate increased from 5.02 to 50.74%, increasing the amounts of short-chain fatty acids (216.61 +/- 2.74 to 1727.55 +/- 23.00
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