文献类型： 外文期刊

作者： Song, Zichao ¹ ; Gao, Yuqing ² ; Yan, Zuqing ¹ ; Zhao, Yanfen ¹ ; Zhang, Aiyu ¹ ; Liu, Fanghua ¹ ; Sun, Jianghua ¹ ; Xu, Yongyu ² ; Tian, Honggang ³ ; Li, Sheng ⁵ ; Wang, Su ⁶ ; Smagghe, Guy ⁷ ; Liu, Tong-Xian ⁷ ; Kang, Zhiwei ¹ ;

作者机构： 1.Hebei Univ, Inst Life Sci & Green Dev, Coll Life Sci, Hebei Basic Sci Ctr Biot Interact, Baoding 071002, Peoples R China

2.Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Peoples R China

3.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

4.Northwest A&F Univ, Key Lab Integrated Pest Management Crops Northwest, Minist Agr & Rural Affairs, Yangling 712100, Shaanxi, Peoples R China

5.South China Normal Univ, Inst Insect Sci & Technol, Sch Life Sci, Guangdong Prov Key Lab Insect Dev Biol & Appl Tech, Guangzhou 510631, Peoples R China

6.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Key Lab Environm Friendly Management Fruit, Beijing 100097, Peoples R China

7.Guizhou Univ, Inst Entomol, Coll Agr, Guiyang 550025, Peoples R China

8.Vrije Univ Brussel VUB, Dept Biol, Cellular & Mol Life Sci, B-1050 Brussels, Belgium

9.Univ Ghent, Fac Biosci Engn, Dept Plants & Crops, B-9000 Ghent, Belgium

关键词： Acyrthosiphon pisum; metamorphosis; cuticle proteins; fecundity; regulation mechanism

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2025 年 45 卷 1 期

页码：

收录情况： SCI

摘要： Kr & uuml;ppel-homolog 1 (Kr-h1) is a key juvenile hormone (JH)-induced transcription factor and plays an important role in insect development and reproduction. However, the molecular function of Kr-h1 in aphids is still unknown. In this study, we used the parthenogenetic pea aphid, Acyrthosiphon pisum, to investigate the presence and role of ApKr-h1 in postembryonic development and reproduction. The tissue distribution and developmental profiles demonstrated that ApKr-h1 was highly expressed in 4th-instar nymph and adults and embryo. Knockdown of ApKr-h1 in 4th-instar nymph resulted in a precocious molting and 79% of aphids were arrested in their molting process. The rest aphids that were able to successfully develop into adults failed to sclerotize normally and died rapidly without producing offspring. Hence, RNA-seq and functional assays revealed that ApKr-h1 can directly regulate the expression of a cuticle protein ApCP53 by binding to its promoter, potentially explaining the failure of molting. Moreover, injection of adults with dsApKr-h1 significantly decreased the fecundity of A. pisum. Our findings not only enrich our knowledge on the function of the Kr-h1 and JH in the development with molting process and reproduction of A. pisum, but may also provide potential targets for RNAi-based management of aphids employing JH-related genes.