TabsPBP2, a Pheromone-Binding Protein Highly Expressed in Male Antennae of Tuta absoluta, Binds Sex Pheromones and Tomato Volatiles
文献类型： 外文期刊
作者： Qu, Cheng 1 ; Yan, Jingxue 1 ; Yan, Zuqing 1 ; Li, Ren 1 ; Liu, Yuqi 1 ; Lin, Aoli 1 ; Fu, Yuejun 3 ; Luo, Chen 1 ; Kang, Zhiwei 4 ; Wang, Ran 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China
2.Beijing Key Lab Environm Friendly Management Fruit, Beijing 100097, Peoples R China
3.Shanxi Univ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Minist Educ, Taiyuan 030006, Peoples R China
4.Hebei Univ, Inst Life Sci & Green Dev, Sch Life Sci, Baoding 071002, Peoples R China
关键词： tomato leafminer; pheromone recognition mechanism; expression profile; fluorescence competition binding assay; molecular docking
期刊名称：BIOMOLECULES （ 影响因子：4.8； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 15 卷 8 期
页码：
收录情况： SCI
摘要： The tomato leafminer (Tuta absoluta), a globally invasive pest, poses a major economic threat to tomato production. Although chemical control remains the primary management method, sustainable alternatives are urgently needed. Sex pheromone communication is critical for moth courtship and mating, with pheromone-binding proteins (PBPs) playing a key role in this process. In this study, we identified a PBP gene, TabsPBP2, from the T. absoluta transcriptome. Real-time quantitative PCR (RT-qPCR) revealed that TabsPBP2 is highly expressed in the antennae, with a strong male-biased expression pattern. Ligand-binding assays demonstrated that TabsPBP2 has the highest affinity for the sex pheromones (3E, 8Z, 11Z)-tetradecatrienyl acetate (TDTA) and (3E, 8Z)-tetradecadienyl acetate (TDDA). It also demonstrated a moderate-to-strong binding affinity to several tomato volatiles, including 2-carene, myrcene, alpha-pinene, cis-3-hexen-l-ol, methyl salicylate, sabinene, and alpha-terpinene. Molecular docking suggested that hydrophobic interactions predominantly stabilize the TabsPBP2-ligand complexes, with PHE118, PHE12, LEU90, LEU68, and ALA73 identified as key interacting residues. Electroantennogram (EAG) and Y-tube olfactometer assays confirmed that TDTA and TDDA act as strong attractants for male T. absoluta. This study enhances our understanding of the pheromone recognition in T. absoluta and provides a foundation for developing novel, pheromone-based pest control strategies.
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