Exogenous nitric oxide generated from SNAP blocks porcine circovirus type 2 replication and regulates NF-kappa B activity in PK-15 cells
文献类型： 外文期刊
作者： Liu, Chuanmin 1 ; Wang, Zhisheng 1 ; Zhang, Qiannan 2 ; Zhang, Bo 2 ; Xue, Tao 2 ; Fu, Jinping 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China
2.Linyi Univ, Sch Med, Linyi, Shandong, Peoples R China
3.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing, Jiangsu, Peoples R China
4.Jiangsu Acad Agr Sci, Key Lab Vet Diag, Nanjing, Jiangsu, Peoples R China
5.Jiangsu Univ, Sch Life Sci, Zhenjiang, Jiangsu, Peoples R China
6.Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China
7.50, Zhongling St, Nanjing 210014, Peoples R China
8.Middle Part Shuangling Rd, Linyi 276000, Peoples R China
关键词： Nitric oxide; SNAP; Porcine circovirus type 2; PK-15 cells; NF-kappa B
期刊名称：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS （ 影响因子：10.8； 五年影响因子：7.2 ）
ISSN： 0924-8579
年卷期： 2023 年 62 卷 2 期
页码：
收录情况： SCI
摘要： Objectives: PCV2-associated disease (PCVAD), caused by porcine circovirus type 2 (PCV2) infection, is one of the major infectious diseases in the global swine industry. Nitric oxide (NO), as an important signalling molecule, has antiviral activities against a variety of viruses. To date, limited knowledge is available on the role of NO during PCV2 infection. Methods: This study was conducted to investigate the effects of exogenous NO on PCV2 replication in vitro. To exclude the possibility that the detected antiviral effects were due to cell toxicity, maximum non-cytotoxic concentrations of the drugs were determined. Kinetics of NO production were assessed after drug treatment. The antiviral activities of NO at different concentrations and at different time points were carefully assessed by measuring the virus titers, viral DNA copies and percentage of PCV2-infected cells. Regulation of NF-kappa B activity by exogenous NO was also investigated. Results: Kinetics of NO production indicated that S-nitroso-acetylpenicillamine (SNAP) produced NO in a dose-dependent manner, while NO was scavenged by its scavenger haemoglobin (Hb). An in vitro antiviral assay demonstrated that exogenous NO strongly inhibited PCV2 replication in a time-dependent and dose-dependent manner, whereas the inhibitory effects could be reversed by Hb. Furthermore, inhibition of NF-kappa B activity induced by NO contributed to a notable decrease in PCV2 replication. Conclusion: These findings provide a new potential antiviral therapy against PCV2 infection, and the antiviral effects of exogenous NO may be partly achieved by regulating NF-kappa B activity. (c) 2023 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.
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