Sensitive and rapid detection of Karenia mikimotoi (Dinophyceae) by loop-mediated isothermal amplification
文献类型: 外文期刊
作者: Zhang, Fengying 1 ; Ma, Lingbo 1 ; Xu, Zhaoli 1 ; Zheng, Junbin 1 ; Shi, Yanhong 1 ; Lu, Yanan 1 ; Miao, Yuping 2 ;
作者机构: 1.Minist Agr China, E China Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Key & Open Lab Marine & Estuarine Fisheries, Shanghai 200090, Peoples R China
2.Minist Agr China, E China Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Key & Open Lab Mar
关键词: identification;karenia mikimotoi;loop-mediated isothermal amplification (LAMP);Ribosomal DNA internal transcribed spacer (ITS)
期刊名称:HARMFUL ALGAE ( 影响因子:4.273; 五年影响因子:5.824 )
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收录情况: SCI
摘要: A loop-mediated isothermal amplification (LAMP) assay was developed to detect the genomic DNA of Karenia mikimotoi using a set of four specific primers based on a ribosomal DNA internal transcribed spacer (ITS). The sensitivity of this LAMP assay was 100-fold higher than regular PCR, and its specificity was validated using other algae as a comparison. Two visual detection approaches were feasible to interpret the positive or negative results. This technology may have the potential to aid in forecasting red-tides on the scene because of its high sensitivity, specificity and rapid detection.
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