Sensitive and rapid detection of Karenia mikimotoi (Dinophyceae) by loop-mediated isothermal amplification
文献类型: 外文期刊
作者: Zhang, Fengying 1 ; Ma, Lingbo 1 ; Xu, Zhaoli 1 ; Zheng, Junbin 1 ; Shi, Yanhong 1 ; Lu, Yanan 1 ; Miao, Yuping 2 ;
作者机构: 1.Minist Agr China, E China Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Key & Open Lab Marine & Estuarine Fisheries, Shanghai 200090, Peoples R China
2.Minist Agr China, E China Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Key & Open Lab Mar
关键词: identification;karenia mikimotoi;loop-mediated isothermal amplification (LAMP);Ribosomal DNA internal transcribed spacer (ITS)
期刊名称:HARMFUL ALGAE ( 影响因子:4.273; 五年影响因子:5.824 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A loop-mediated isothermal amplification (LAMP) assay was developed to detect the genomic DNA of Karenia mikimotoi using a set of four specific primers based on a ribosomal DNA internal transcribed spacer (ITS). The sensitivity of this LAMP assay was 100-fold higher than regular PCR, and its specificity was validated using other algae as a comparison. Two visual detection approaches were feasible to interpret the positive or negative results. This technology may have the potential to aid in forecasting red-tides on the scene because of its high sensitivity, specificity and rapid detection.
- 相关文献
作者其他论文 更多>>
-
Screening and Analysis of Potential Aquaculture Spaces for Larimichthys crocea in China's Surrounding Waters Based on Environmental Temperature Suitability
作者:Yang, Ling;Zhou, Weifeng;Cui, Xuesen;Lu, Yanan;Liu, Qin;Yang, Ling
关键词:
Larimichthys crocea ; deep-sea aquaculture; potential spaces; spatial analysis; China -
COMPARATIVE ANALYSIS OF THE GROWTH PERFORMANCE IN THE PORTUNIDAE) UNDER DIFFERENT SEX RATIOS PROVIDES NEW INSIGHTS FOR CRAB AQUACULTURE: A LABORATORY STUDY
作者:Luo, Minghao;Jin, Xin;Ma, Lingbo;Luo, Minghao;Wang, Xueyang;Liu, Zhiqiang;Jin, Xin;Wang, Wei;Ma, Lingbo;Zhao, Ming
关键词:Scylla paramamosain; aquaculture; sex ratio; growth performance; survival ratio
-
Integration of metagenome and full-length 16S rRNA gene sequencing of the mariculture environmental bacterial community reveal potential pathogenic bacteria in broodstock breeding of the mud crab, Scylla paramamosain
作者:Xu, Likun;Hu, Xin;Ma, Chunyan;Wang, Wei;Zhang, Fengying;Zhao, Ming;Ma, Lingbo;Ma, Keyi;Liu, Zhiqiang;Xu, Jiayuan;Lv, Xiaokang;Su, Zhixing
关键词:Scylla paramamosain; Bacteria; Metagenomic sequencing; Full-length 16S rRNA; Pathogenic bacteria
-
An Efficient Downwelling Light Sensor Data Correction Model for UAV Multi-Spectral Image DOM Generation
作者:Wu, Siyao;Lu, Yanan;Fan, Wei;Zhang, Shengmao;Wu, Zuli;Wang, Fei
关键词:rotary-wing UAV; DLS; reflectance; curve fitting
-
Comparative analysis of phytoplankton community structure and stability in the Arabian Sea and Bay of Bengal using eDNA metabarcoding
作者:Li, Changwen;Xiao, Ge;Ai, Tongxi;Tang, Fenghua;Ju, Jianfeng;Yang, Shenglong;Gao, Lei;Ma, Lingbo;Fan, Wei;Zhao, Ming;Zheng, Hanfeng;Jiang, Keji;Li, Changwen;Ai, Tongxi;Ju, Jianfeng;Long, Han;Pi, Yan
关键词:eDNA; AS; BB; Phytoplankton; Diversity; Stability
-
THE EFFECT OF DIFFERENT SALINITIES ON THE GILL MORPHOLOGY AND RELATED GENE EXPRESSION OF LARVAL SCYLLA PARAMAMOSAIN ESTAMPADOR, 1950 (BRACHYURA, PORTUNIDAE)
作者:Yin, Chunyan;Shi, Fangfang;Liu, Zhiqiang;Zhao, Ming;Ma, Chunyan;Ma, Lingbo;Wang, Wei;Zhang, Fengying;Yin, Chunyan;Shi, Fangfang
关键词:Salinity; gene expression; Na plus -K plus -Cl--cotransporter (NKCC); carbonic anhydrase (CA)
-
Feeding rhythm of the zoea larvae of Scylla paramamosain: The dynamic feeding rhythm is not completely synchronized with photoperiod
作者:Wang, Wei;Liu, Zhiqiang;Wang, Xueyang;Zhang, Fengying;Ma, Chunyan;Zhao, Ming;Ma, Keyi;Ma, Lingbo
关键词:Scylla paramamosain; Zoea; Feeding rhythm; Feeding rate; Digestive enzyme



