文献类型： 外文期刊
作者： Kong, Delong 1 ; Xia, Zhanfeng 1 ; Gao, Yan 3 ; Zhou, Yiqing 2 ; Jiang, Xu 2 ; Xu, Piao 4 ; Guo, Wei 5 ; Ruan, Zhiyong 2 ;
作者机构： 1.Tarim Univ, Coll Life Sci, Key Lab Protect & Utilizat Biol Resources Tarim B, Alar 843300, Peoples R China
2.CAAS, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China
3.Xinjiang Acad Agr Sci Xinjiang Special Environm M, Inst Appl Microbiol, Urumqi 830091, Peoples R China
4.Guangxi Jiandibao Biotechnol Ltd, Nanning 530105, Peoples R China
5.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
6.Tibet Agr & Anim Husb Univ, Coll Resources & Environm, Linzhi 860000, Tibet, Peoples R China
关键词： Sporosarcina; draft genome; phylogenetic analysis; polyphasic taxonomy
期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.689； 五年影响因子：2.925 ）
ISSN： 1466-5026
年卷期： 2022 年 72 卷 2 期
页码：
收录情况： SCI
摘要： A Gram-stain-positive, aerobic, motile, rod-shaped bacterium, designated strain LAM9210(T). was isolated from a saline soil sample collected from Lingxian County. Shandong Province, PR China. Analysis of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strain of Sporosarcina pasteurii NCIMB 8841(T) (97.6% sequence similarity). The genomic G+C content was 40.4 mol%. The average nucleotide identity and in silico DNA-DNA hybridization values between strain LAM9210(T) and the type strain of the most closely related species S. pasteurii NCIMB 8841(T) were 73.6 and 20.6%, respectively. Strain LAM9210(T) was found to grow at 10-40 degrees C (optimum, 30 degrees C), at pH 6.0-10.0 (optimum, pH 9.0) and with 0-6% (w/v) NaCl (optimum. 0.5%), respectively. The major fatty acids were anteiso-C-15 : 0 and iso-C-14 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid. Menaquinone-7 was detected as the predorminant respiratory quinone. Strain LAM9210(T) contained glycine. lysine, alanine and glutamic acid as the diagnostic amino acids in the cell-wall peptidoglycan. On the basis of phenotypic, phylogenetic and genotypic data, strain LAM9210(T) is considered to represent a novel species of the genus Sporosarcina, for which the name Sporosarcina jiandibaonis sp. nov. is proposed. The type strain is LAM9210(T) (=CGMCC 1.18607(T)=GDMCC 1.2002(T)=JCM 32514(T)).
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