Promotion of nicotine biosynthesis in transgenic tobacco by overexpressing allene oxide cyclase from Hyoscyamus niger
文献类型： 外文期刊
作者： Jiang, Keji 2 ; Pi, Yan 2 ; Hou, Rong 2 ; Jiang, Lili 4 ; Sun, Xiaofen 2 ; Tang, Kexuan 1 ;
作者机构： 1.Shanghai Jiao Tong Univ, Shanghai Key Lab Agrobiotechnol, Plant Biotechnol Res Ctr, Sch Agr & Biol,Inst Syst Biol,Fudan SJTU Nottingh, Shanghai 200030, Peoples R China
2.Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci,Fudan SJTU Nottingham Plant Biotechn, Shanghai 200433, Peoples R China
3.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
4.Shanghai Putuo Municipal Ctr Dis Control & Preven, Shanghai 20033, Peoples R China
关键词： Allene oxide cyclase;Hyoscyamus niger;Jasmonate;Metabolic engineering;Metabolite;Nicotiana tabacum;Nicotine;Transformation
期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Plant secondary metabolites are a wide variety of low-molecular weight compounds whose productions are often enhanced in response to both biotic and abiotic stresses. Many of the responses are mediated by a class of hormones, named as jasmonates. In jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most crucial step. Here a heterologous AOC gene from Hyoscyamus niger L. (black henbane), named HnAOC (GenBank accession No. AY708383), was overexpressed in Nicotiana tabacum cv. Petit Havana to investigate the consequence on nicotine content. This study revealed that the transcription of HnAOC in tobacco resulted in overexpression of nicotine biosynthetic pathway genes and higher yield of nicotine, with the maximum of 4.8-fold over control. Therefore, it indicated that without the cost of extrinsic hormones, genetic manipulation of jasmonate biosynthetic pathway genes could be an alternative approach in metabolic engineering for the production of valuable secondary metabolites, which were induced by jasmonates.
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