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Immobilization of Chitosanase on Magnetic Nanoparticles: Preparation, Characterization and Properties

文献类型： 外文期刊

作者： Cheng Yimeng 1 ; Li Zhaoyue 2 ; Sun Huihui 2 ; Zhao Ling 2 ; Liu Zhen 1 ; Cao Rong 2 ; Liu Qi 2 ; Mao Xiangzhao 1 ;

作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Dept Food Engn & Nutr, Qingdao 266071, Peoples R China

3.Lab Marine Drugs & Bioprod Qingdao, Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China

关键词： chitosanase; immobilization; magnetic nanoparticles; chitooligosaccharides

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：1.179； 五年影响因子：1.225 ）

ISSN： 1672-5182

年卷期： 2022 年 21 卷 5 期

页码：

收录情况： SCI

摘要： Chitosanase could cleave beta-1,4-linkage of chitosan to produce chitooligosaccharides (COS) with diverse biological activities. However, there are many limitations on the use of free chitosanase in industrial production. Enzyme immobilization is generally considered a valuable strategy in industrial-scale applications. In this study, the chitosanase Csn-BAC from Bacillus sp. MD-5 was immobilized on Fe3O4-SiO2 magnetic nanoparticles (MNPs) to enhance its properties, which could be recovered easily from reaction media using magnetic separation. The activities of Csn-BAC immobilized with MNPs (MNPs@Csn-BAC) were determined with temperature and pH, and the thermal- and pH-stabilities, respectively. The reusability of the MNPs@Csn-BAC was determined in repeated reaction cycles. Immobilization enhanced the thermal and pH stability of Csn-BAC compared with the free enzyme. After eight reaction cycles using MNPs@Csn-BAC, the residual enzyme activity was 72.15%. Finally, the amount of COS released by MNPs@Csn-BAC was 1.86 times higher than that of the free Csn-BAC in the catalytic performance experiment. The immobilized Csn-BAC exhibits broad application prospects in the production of COS.

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