Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences
文献类型： 外文期刊
作者： Diao, Y. 1 ; Miao, Y. 1 ; Lin, X. 3 ; Liao, C. 3 ; Guo, F. 4 ; Hu, Z. 1 ;
作者机构： 1.Wuhan Univ, Coll Life Sci, Minist Educ Plant Dev Biol, Key Lab, Wuhan 430072, Peoples R China
2.Chongqing Univ Arts & Sci, Coll Life Sci & Technol, Yongchuan 402168, Peoples R China
3.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi 445000, Peoples R China
4.Yunnan Agr Univ, Perilla Res Inst, Kunming 650201, Peoples R China
关键词： GENETIC-RELATIONSHIPS;WEEDY TYPES;MARKERS
期刊名称：RUSSIAN JOURNAL OF GENETICS （ 影响因子：0.581； 五年影响因子：0.729 ）
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收录情况： SCI
摘要： In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety.
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