文献类型： 外文期刊

作者： Sun, Rujian ¹ ; Sun, Bincheng ³ ; Tian, Yu ² ; Su, Shanshan ⁴ ; Zhang, Yong ⁵ ; Zhang, Wanhai ³ ; Wang, Jingshun ³ ; Yu, Ping ³ ; Guo, Bingfu ² ; Li, Huihui ² ; Li, Yanfei ² ; Gao, Huawei ² ; Gu, Yongzhe ² ; Yu, Lili ² ; Ma, Yansong ² ; Su, Erhu ⁶ ; Li, Qiang ⁶ ; Hu, Xingguo ³ ; Zhang, Qi ³ ; Guo, Rongqi ³ ; Chai, Shen ³ ; Feng, Lei ³ ; Wang, Jun ² ; Hong, Huilong ² ; Xu, Jiangyuan ² ; Yao, Xindong ⁷ ; Wen, Jing ² ; Liu, Jiqiang ⁴ ; Li, Yinghui ¹ ; Qiu, Lijuan ¹ ;

作者机构： 1.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, 12 Zhongguancun South St, Beijing 100081, Peoples R China

3.Hulunbuir Inst Agr & Anim Husb, Hulunbuir 021000, Peoples R China

4.Beijing Compass Biotechnol Co Ltd, Beijing 102200, Peoples R China

5.Heilongjiang Acad Agr Sci, Keshan Branch, Qiqihar 161600, Peoples R China

6.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010000, Peoples R China

7.Univ Nat Resources & Life Sci Vienna BOKU, Dept Crop Sci, A-3430 Tulln, Austria

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 4 期

页码：

收录情况： SCI

摘要： Key message We developed the ZDX1 high-throughput functional soybean array for high accuracy evaluation and selection of both parents and progeny, which can greatly accelerate soybean breeding. Microarray technology facilitates rapid, accurate, and economical genotyping. Here, using resequencing data from 2214 representative soybean accessions, we developed the high-throughput functional array ZDX1, containing 158,959 SNPs, covering 90.92% of soybean genes and sites related to important traits. By application of the array, a total of 817 accessions were genotyped, including three subpopulations of candidate parental lines, parental lines and their progeny from practical breeding. The fixed SNPs were identified in progeny, indicating artificial selection during the breeding process. By identifying functional sites of target traits, novel soybean cyst nematode-resistant progeny and maturity-related novel sources were identified by allele combinations, demonstrating that functional sites provide an efficient method for the rapid screening of desirable traits or gene sources. Notably, we found that the breeding index (BI) was a good indicator for progeny selection. Superior progeny were derived from the combination of distantly related parents, with at least one parent having a higher BI. Furthermore, new combinations based on good performance were proposed for further breeding after excluding redundant and closely related parents. Genomic best linear unbiased prediction (GBLUP) analysis was the best analysis method and achieved the highest accuracy in predicting four traits when comparing SNPs in genic regions rather than whole genomic or intergenic SNPs. The prediction accuracy was improved by 32.1% by using progeny to expand the training population. Collectively, a versatile assay demonstrated that the functional ZDX1 array provided efficient information for the design and optimization of a breeding pipeline for accelerated soybean breeding.