GhWRKY40 Interacts with an Asparaginase GhAPD6 Involved in Fiber Development in Upland Cotton (Gossypium hirsutum L.)
文献类型： 外文期刊
作者： Zhang, Sujun 1 ; Cai, Xiao 1 ; Wei, Jingyan 3 ; Wang, Haitao 1 ; Liu, Cunjing 1 ; Li, Xinghe 1 ; Tang, Liyuan 1 ; Zhou, Xiaodong 1 ; Zhang, Jianhong 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cotton, Shijiazhuang 050051, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Cotton Huanghuaih, Shijiazhuang 050051, Peoples R China
3.Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Peoples R China
关键词： fiber quality; GhWRKY40; aspartyl protease; yeast two-hybrid assay; split-luciferase complementation assay; Gossypium hirsutum
期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN：
年卷期： 2024 年 15 卷 8 期
页码：
收录情况： SCI
摘要： Fiber quality improvement is a primary goal in cotton breeding. Identification of fiber quality-related genes and understanding the underlying molecular mechanisms are essential prerequisites. Previously, studies determined that silencing the gene GhWRKY40 resulted in longer cotton fibers; however, both the underlying mechanisms and whether this transcription factor is additionally involved in the regulation of cotton fiber strength/fineness are unknown. In the current study, we verified that GhWRKY40 influences the fiber strength, fiber fineness, and fiber surface structure by using virus-induced gene silencing (VIGS). Potential proteins that may interact with the nucleus-localized GhWRKY40 were screened in a yeast two-hybrid (Y2H) nuclear-system cDNA library constructed from fibers at 0, 10, and 25 days post-anthesis (DPA) in two near-isogenic lines differing in fiber length and strength. An aspartyl protease/asparaginase-related protein, GhAP(D6), was identified and confirmed by Y2H and split-luciferase complementation assays. The expression of GhAP(D6) was approximately 30-fold higher in the GhWRKY40-VIGS lines at 10 DPA and aspartyl protease activity was significantly upregulated in the GhWRKY40-VIGS lines at 10-20 DPA. This study suggested that GhWRKY40 may interact with GhAP(D6) to regulate fiber development in cotton. The results provide a theoretical reference for the selection and breeding of high-quality cotton fibers assisted by molecular technology.
- 相关文献
作者其他论文 更多>>
-
MYB regulation of GST/GT mediates red petal spot development in cotton
作者：Zhang, Sujun;Wang, Haitao;Cai, Xiao;Liu, Cunjing;Tang, Liyuan;Li, Xinghe;Zhang, Jianhong;Chen, Jie;Jiang, Tao;Sun, Yaqian
关键词：Cotton; Red-spot-petal; MYB-encoding gene; Glutathione S transferase gene; Glycosyl-transferase gene
-
A plastid lipid-associated protein-encoding gene (GhPAP) that positively regulates fiber strength was identified via genetic mapping and transcriptomic analysis of a stable QTL on chromosome D06 of upland cotton
作者：Zhang, Sujun;Liu, Cunjing;Tang, Liyuan;Li, Xinghe;Cai, Xiao;Wang, Haitao;Zhang, Jianhong;Zhang, Jinfa
关键词：
-
Identification of the cysteine-rich transmembrane module CYSTM family in upland cotton and functional analysis of GhCYSTM5_A in cold and drought stresses
作者：Cai, Xiao;Tang, Liyuan;Wang, Haitao;Zhang, Sujun;Li, Xinghe;Liu, Cunjing;Zhang, Xiangyun;Zhang, Jianhong
关键词：CYSTM; Cold stress; Drought stress; Gossypium hirsutum
-
GhDLDH1 up-regulates synthesis of chloroplast responding to salinity stress in Gossypium hirsutum L.
作者：Fan, Yapeng;Wang, Shuai;Huang, Hui;Chen, Xiugui;Wang, Junjuan;Zhao, Lanjie;Guo, Lixue;Sun, Yuping;Yang, Zhining;Ye, Wuwei;Liu, Yi;Qian, Yuyuan;Zhang, Xi;Li, Shaoqi;Wang, Guangen;Zhang, Jianhong
关键词：D-lactate dehydrogenase; Chloroplast; Salt tolerance; Gossypium hirsutum L
-
Dynamic Metabolite Profile Changes in Semen Ziziphi Spinosae During Ripening
作者：Tian, Xi;Tian, Wei;Liu, Lingdi;Jiang, Tao;Zhang, Sujun;Gu, Liqiang;Li, Qiang
关键词：different harvest times; metabolite;
Semen Ziziphi Spinosae ; UPLC-Q Trap-MS/MS -
A cysteine-rich transmembrane module peptide GhCYSTM9 is involved in cold stress response
作者：Cai, Xiao;Liu, Cunjing;Tang, Liyuan;Zhang, Sujun;Li, Xinghe;Wang, Haitao;Zhang, Jianhong
关键词：
GhCYSTM9 ; Cysteine-rich peptide; Cold stress;Gossypium hirsutum -
Aspartyl proteases identified as candidate genes of a fiber length QTL, qFLD05, that regulates fiber length in cotton (Gossypium hirsutum L.)
作者：Zhang, Sujun;Wang, Haitao;Li, Xinghe;Tang, Liyuan;Cai, Xiao;Liu, Cunjing;Zhang, Xiangyun;Zhang, Jianhong
关键词：
