Cell-penetrating peptides-modified nanocarrier for efficient, batch and noninvasive embryonic transfection
文献类型： 外文期刊
作者： Li, Yundong 1 ; Huang, Sheng 1 ; Zheng, Yunsheng 2 ; Xie, Jiancong 2 ; Jiang, Shigui 1 ; Jiang, Song 1 ; Huang, Jianhua 1 ; Yang, Lishi 1 ; Yang, Qibin 1 ; Shi, Jianzhi 1 ; Ding, Yangyang 1 ; Li, Erchao 3 ; Cao, Ziyang 2 ; Zhou, Falin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploita, Guangzhou 510300, Peoples R China
2.South China Univ Technol, Affiliated Hosp 2, Sch Med, Guangzhou 510180, Guangdong, Peoples R China
3.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China
4.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572018, Peoples R China
关键词： Nanocarrier; Gene editing; CRISPR/Cas9; Aquatic animal; Prawn
期刊名称：COLLOIDS AND SURFACES B-BIOINTERFACES （ 影响因子：5.6； 五年影响因子：5.4 ）
ISSN： 0927-7765
年卷期： 2025 年 255 卷
页码：
收录情况： SCI
摘要： Although gene-editing breeding demonstrates significant potential in aquaculture, the field still lacks efficient gene delivery systems. Fish and crustacean embryos typically possess hard egg membranes or thick shells, making conventional microinjection inefficient and causing high mortality rates. As a result, there is an urgent need for a universal, highly efficient, and low-toxicity noninvasive delivery platform. In this study, we investigated the potential of high-efficiency, large-scale transfection using nanocarrier technology, renowned for its biocompatibility and efficient molecular payload encapsulation. Our research focused on developing a novel nanocarrier system, termed TNP, composed of a poly(ethylene glycol)-poly(lactide-co-glycolide) block copolymer (PEG-b-PLGA) and a cell-penetrating peptide (TAT, transactivator of transcription). The TNP nanocarrier enabled noninvasive delivery of genetic cargo, including eGFP-mRNA and CRISPR/Cas9 components, achieving high-throughput transfection in both shrimp and zebrafish embryos with successful transgene expression and targeted gene editing. We demonstrated that TNP efficiently traversed embryonic barriers, leveraging to facilitate high-payload gene delivery while maintaining minimal cytotoxicity and high embryo viability based on the transmembrane functionality of TAT peptide. These findings highlight the TNP has the potential to transform aquaculture genetic engineering, enabling trait enhancement such as disease resistance and accelerated growth. Furthermore, this study establishes a versatile and efficient gene-editing platform for aquatic species, addressing the critical demand for sustainable and ethically responsible biotechnological innovations in global seafood production.
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