Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis
文献类型： 外文期刊
作者： Gao, Z. Q. 2 ; Meng, C. X. 2 ; Wang, G. C. 1 ; Ye, N. H. 3 ;
作者机构： 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
关键词： Porphyra yezoensis;photosytem II;filamentous sporophytes;thylakoid membranes;polypeptide composition;sucrose density gradient ultracentrifugation
期刊名称：INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS （ 影响因子：1.918； 五年影响因子：1.406 ）
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收录情况： SCI
摘要： Thylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the photosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC. The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein was also detected. However, cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.
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