Transcriptomic Dynamics of Rice Varieties with Differential Cold Tolerance Under Low-Temperature Stress During Grain-Filling Stage
文献类型： 外文期刊
作者： Cao, Liangzi 1 ; Wang, Xueyang 3 ; Liu, Yingying 4 ; Ding, Guohua 1 ; Zhou, Jinsong 1 ; Lei, Lei 1 ; Bai, Liangming 1 ; Luo, Yu 1 ; Sun, Shichen 1 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Inst Tillage & Cultivat, Harbin 150088, Peoples R China
2.Heilongjiang Rice Qual Improvement & Genet Breedin, Harbin 150086, Peoples R China
3.Heilongjiang Acad Agr Sci, Soybean Res Inst, Harbin 150086, Peoples R China
4.Northeast Agr Univ, Agr Coll, Harbin 150000, Peoples R China
关键词： rice; cold stress; grain-filling stage; taste quality; starch and sucrose metabolism; SUS
期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 16 卷 8 期
页码：
收录情况： SCI
摘要： Background/Objectives: Low-temperature stress during the grain-filling stage negatively affects rice grain quality and yield. Understanding the physiological and molecular mechanisms underlying cold tolerance is critical for breeding rice varieties with improved resilience. Methods: In this study, eight rice varieties with differential cold tolerance-LD1603, 13108, LD18, and 4-1021 (cold-tolerant) and LD3, LD4, LD121, and LD1604 (cold-sensitive)-were subjected to 17.5 degrees C low-temperature stress during grain filling in a naturally illuminated phytotron. Amylose and protein content, as well as taste quality, were analyzed. RNA sequencing was performed to identify differentially expressed genes and transcription factors associated with cold response. Results: Under low-temperature stress, amylose and protein content significantly increased in all eight varieties. The taste quality of cold-sensitive varieties declined markedly, whereas cold-tolerant varieties maintained higher and more stable taste quality values. Transcriptomic analysis revealed that key enzyme genes (INV, SUS, HXK, FRK, amyA, and TPP) in the starch and sucrose metabolism pathway were significantly upregulated in cold-tolerant varieties (LD18 and 4-1021), but suppressed in cold-sensitive varieties. Several cold-responsive transcription factors from the NAC, WRKY, AP2/ERF, MYB, and bZIP families were also identified. Weighted gene co-expression network analysis (WGCNA) further revealed hub TFs (OsWRKY1, OsWRKY24, OsWRKY53, and OsMYB4) and structural genes (OsPAL04 and OsCDPK7) potentially involved in cold tolerance during grain filling. Conclusions: This study enhanced our understanding of the molecular response to low temperature during rice grain filling and provided candidate genes for developing cold-tolerant rice varieties through molecular breeding.
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