文献类型： 外文期刊

作者： Wu, Wangze ¹ ; Yang, Haobo ² ; Ding, Haijun ¹ ; Zhu, Guoting ¹ ; Xing, Peng ¹ ; Wu, Yujun ⁴ ; Han, Xueyan ¹ ; Xue, Mei ¹ ; Shen, Juan ⁵ ; Dong, Yun ⁶ ;

作者机构： 1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China

2.Guangzhou Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Adaptat & Mol Design, Guangzhou 510006, Peoples R China

3.Gansu Agr Univ, Coll Agron, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

4.Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Xining 810016, Peoples R China

5.Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Peoples R China

6.Gansu Acad Agr Sci, Crop Res Inst, Lanzhou 730070, Peoples R China

关键词： BrRLCK1; Brassica rapa; Receptor-like cytoplasmic kinase; Freezing tolerance; Phylogenetic tree; CBF pathway

期刊名称：GENE （ 影响因子：2.4； 五年影响因子：2.8 ）

ISSN： 0378-1119

年卷期： 2025 年 941 卷

页码：

收录情况： SCI

摘要： Some winter rapeseed (Brassica rapa) varieties can endure extremely low temperatures (-20 degrees C to-32 degrees C). However, because of a lack of mutant resources, the molecular mechanisms underlying cold tolerance in B. rapa remain unclear. In this study, we identified a low-temperature-sensitive mutant receptor-like cytoplasmic kinase (RLCK), BrRLCK1, using the B. rapa-Arabidopsis (Arabidopsis thaliana) full-length cDNA-overexpressing gene hunting system mutant library. BrRLCK1, localized to the plasma membrane and retained its localization under low temperatures. Phylogenetic analysis showed that BrRLCK1 is highly conserved across six widely cultivated Brassica species, but exhibits complexity due to genome hybridization and polyploidization. Notably, (3-glucuronidase activity and qRT-PCR analysis showed that B. rapa BrRLCK1 and its homologous gene BrRLCK2 were mainly expressed in the main root, shoot, and leaves, with their expression being activated by low temperatures. Transgenic Arabodipsis expressing BrRLCK1 and BrRLCK2 reduced freezing tolerance and promoted root elongation. These combined results indicated that low temperatures can activate the expression of BrRLCK1 and BrRLCK2, negatively regulating freezing tolerance via the C-repeat-binding factor (CBF) pathway.