文献类型： 外文期刊

作者： Sun, Liping ¹ ; Shi, Fengfeng ¹ ; He, Xingjiang ³ ; Cai, Yujiao ¹ ; Yu, Yinglong ³ ; Yao, Dan ³ ; Zhou, Jinhui ¹ ; Wei, Xiaoping ³ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Bee Prod Qual & Safety Control, Beijing 100093, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Inst Integrated Agr Dev, Guiyang 550006, Peoples R China

关键词： Unifloral honey; Short chain organic acids; Solid-phase extraction; Derivatization; SPE-GC-MS

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：3.498； 五年影响因子：3.455 ）

ISSN： 1438-2377

年卷期：

页码：

收录情况： SCI

摘要： Honey, a natural healthy liquid bee product, is rich in amino acids, vitamins, and other essential nutrients. Different origin honeys also varied in organic acids. The objective of this study is to establish an efficient solid-phase extraction-gas chromatography-mass spectrometry (SPE-GC-MS) method to eliminate interference of sugar and other impurities for accurately estimating 22 organic acids in honey by optimizing extraction, purification, derivatization, and gas chromatography-mass spectrometry (GC-MS) analytical conditions. After being extracted, purified and derivatized, organic acids in honey were qualified and quantified by GC-MS. This method was evaluated experimentally, and the results showed that, within a certain concentration range, the standard curve linear relationship was satisfactory (R-2 > 0.9942), and the target organic acid recovery rate was 86.74%similar to 118.68%. Besides, precision (relative standard deviation, RSD= 2.98%similar to 13.42%), detection limit (LOD = 0.002 similar to 0.2 mg kg(-1)), and quantification limit (LOQ = 0.008 similar to 0.5 mg kg(-1)) met the target requirements. Also, based on this analytical method, the organic acids in five types of honey (acacia, jujube, vitex, canola and linden honey) were estimated. Notably, they all contained 22 different kinds of organic acids, and significant differences (p < 0.05) in the organic acid content and composition among different honey varieties were observed. PCA analysis showed that the five honeys could be differentiated based on the content of 22 organic acids.