Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in alpha-Linolenic Acid Biosynthesis in Perilla Seeds
文献类型： 外文期刊
作者： Wu Duan 1 ; Yang Shi-Mei 1 ; Shang Zhi-Wei 3 ; Xu, Jing 3 ; Zhao De-Gang 2 ; Wang Hong-Bin 1 ; Shen, Qi 1 ;
作者机构： 1.Guangzhou Univ Chinese Med, Inst Med Plant Physiol & Ecol, Sch Pharmaceut Sci, Guangzhou, Peoples R China
2.Guizhou Univ, Coll Life Sci, Key Lab Plant Resource Conservat & Germplasm Inno, Guiyang, Peoples R China
3.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang, Peoples R China
关键词： perilla; alpha-linolenic acid (C18:3); fatty acid desaturases; genome-wide analysis; plant transformation
期刊名称：FRONTIERS IN GENETICS （ 影响因子：3.7； 五年影响因子：4.3 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Perilla (Perilla frutescens), a traditional medicinal and oilseed crop in Asia, contains extremely high levels of polyunsaturated alpha-linolenic acid (ALA) (up to 60.9%) in its seeds. ALA biosynthesis is a multistep process catalyzed by fatty acid desaturases (FADs), but the FAD gene family in perilla has not been systematically characterized. Here, we identified 42 PfFADs in the perilla genome and classified them into five subfamilies. Subfamily members of PfFADs had similar exon/intron structures, conserved domain sequences, subcellular localizations, and cis-regulatory elements in their promoter regions. PfFADs also possessed various expression patterns. PfFAD3.1 was highly expressed in the middle stage of seed development, whereas PfFAD7/8.3 and PfFAD7/8.5 were highly expressed in leaf and later stages of seed development, respectively. Phylogenetic analysis revealed that the evolutionary features coincided with the functionalization of different subfamilies of PUFA desaturase. Heterologous overexpression of PfFAD3.1 in Arabidopsis thaliana seeds increased ALA content by 17.68%-37.03%. These findings provided insights into the characteristics and functions of PfFAD genes in perilla.
- 相关文献
作者其他论文 更多>>
-
Rosa roxburghii Tratt Pomace Crude Extract Inactivates Cronobacter sakazakii Isolated from Powdered Infant Formula
作者：Fei, Peng;Xu, Jing;Xie, Jinlan;Huang, Jicheng;Jiang, Peiyi;Guo, Mingliang;Feng, Hongxia;Chang, Yunhe;Chen, Xi
关键词：Rosa roxburghii Tratt pomace crude extract; Cronobacter sakazakii; inactivation effect; natural disinfectant; contact surface
-
Incipient diploidization of the medicinal plant Perilla within 10,000 years
作者：Zhang, Yujun;Shen, Qi;Leng, Liang;Zhang, Dong;Chen, Sha;Shi, Yuhua;Chen, Shilin;Shen, Qi;Ning, Zemin;Shen, Qi
关键词：
-
Comparative transcriptome and co-expression analysis reveal key genes involved in leaf margin serration in Perilla frutescens
作者：Shen, Qi;Shen, Qi;Zhang, Dong;Zhang, Tian-yuan;Shen, Qi;Zhao, De-gang;Xu, Yang-yang
关键词：leaf shape development; Perilla frutescens (L.) Britt; transcriptome sequencing; wrinkly leaves
-
RNA Sequencing and Coexpression Analysis Reveal Key Genes Involved in -Linolenic Acid Biosynthesis in Perilla frutescens Seed
作者：Zhang, Tianyuan;Shang, Zhiwei;Yang, Sen;Shen, Qi;Zhao, Degang;Zhang, Tianyuan;Song, Li;Zhao, Degang;Song, Chi;Zhang, Dong;Sun, Wei
关键词：Perilla frutescens;RNA sequencing (RNA-seq);-linolenic acid (ALA);triacylglycerol (TAG) biosynthesis;herbgenomics
-
Five Fatty Acyl-Coenzyme A Reductases Are Involved in the Biosynthesis of Primary Alcohols in Aegilops tauschii Leaves
作者：Wang, Meiling;Wu, Hongqi;Li, Chunlian;Wang, Yong;Wang, Zhonghua;Xu, Jing
关键词：fatty acyl-coenzyme A reductase;primary alcohol;cuticular wax;Saccharomyces cerevisiae;Aegilops tauschii;Triticum aestivum
-
Genome-scale identification of MLO domain-containing genes in soybean (Glycine max L. Merr.)
作者：Shen, Qi;Zhao, Jinming;Xiang, Yang;Shen, Qi;Du, Caifu;Xiang, Yang;Qin, Xinrong;Cao, Jinxuan
关键词：soybean;MLO domain;gene family;abiotic stress;phytohormone
-
Proteomics study of changes in soybean lines resistant and sensitive to Phytophthora sojae
作者：Zhang, YuMei;Zhao, JinMing;Bian, XiaoChun;Zuo, QiaoMei;Gai, JunYi;Xing, Han;Xiang, Yang;Shen, Qi
关键词：
