Transcriptome Analysis Reveals the Regulatory Mechanism of Lipid Metabolism and Oxidative Stress in Litopenaeus vannamei under Low-Salinity Stress
文献类型： 外文期刊
作者： Cao, Siyao 1 ; Li, Yundong 1 ; Jiang, Song 1 ; Yang, Qibin 1 ; Huang, Jianhua 1 ; Yang, Lishi 1 ; Shi, Jianzhi 1 ; Jiang, Shigui 1 ; Wen, Guoliang 1 ; Zhou, Falin 1 ;
作者机构： 1.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572018, Peoples R China
2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
4.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518108, Peoples R China
关键词： Litopenaeus vannamei; salinity stress; transcriptome analysis; differentially expressed genes; peroxisome
期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.8； 五年影响因子：2.8 ）
ISSN：
年卷期： 2024 年 12 卷 8 期
页码：
收录情况： SCI
摘要： Salinity is a crucial environmental factor influencing the survival, growth, development, and reproduction of aquatic animals. However, the underlying molecular mechanisms of the shrimp's response to salinity stress are not yet fully understood. Therefore, we used the Illumina NovaSeq 6000 platform to perform transcriptome sequencing of the hepatopancreas of Litopenaeus vannamei under high-salinity (30 PSU), medium-salinity (10 PSU), and low-salinity (0.5 PSU) conditions. We obtained 63.23 Gb of high-quality data and identified 3589 differentially expressed genes (DEGs), including 1638 upregulated and 1951 downregulated genes. Notably, a comparison between the control group (30 PSU) and the low-salinity group (0.5 PSU) revealed that the BBOX1 and CHE1 genes were significantly upregulated, while the ACOX1, MPV, CYP2L1, GCH, MVK, TREt1, and XDH genes were significantly downregulated. These genes are primarily involved in key metabolic pathways, such as fatty acid oxidation, cholesterol metabolism, and hormone synthesis and metabolism. The key genes involved in fatty acid beta-oxidation, such as ACOX1, ACAD, HADH, HSD17B4, PECR, CROT, PIPOX, and CG5009, all showed a downward trend, suggesting that L. vannamei may respond to salt stress by regulating fatty acid oxidative metabolism, optimizing energy utilization, and maintaining cell homeostasis under low-salinity conditions. Functional annotation of gene ontology (GO) and KEGG pathway enrichment analysis highlighted the roles of these significant DEGs in the adaptation of L. vannamei to environments of varying salinity, underscoring the importance of metabolic pathways in their adaptive physiological responses. This study provides a crucial molecular biological basis for understanding the molecular mechanisms and physiological protection strategies of L. vannamei under salinity stress.
- 相关文献
作者其他论文 更多>>
-
Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds
作者：Jing, Lu;Wen, Guoliang;Cao, Yucheng;Jing, Lu;Ren, Huan;Xu, Wujie;Su, Haochang;Hu, Xiaojuan;Wen, Guoliang;Xu, Yu;Tu, Lingjie;Cao, Yucheng;Xu, Wujie;Su, Haochang;Hu, Xiaojuan;Wen, Guoliang;Cao, Yucheng;Xu, Yu
关键词：Litopenaeus vannamei; Water quality; Biofloc bacteria; Growth performance; Gene expression
-
Metabolic Response of Black Tiger Shrimp (Penaeus monodon) to Acute Ammonia Nitrogen Stress
作者：Ding, Yangyang;Jiang, Shigui;Jiang, Song;Li, Yundong;Yang, Qibin;Yang, Lishi;Shi, Jianzhi;Li, Pengying;Diao, Hongshan;Zhou, Falin;Ding, Yangyang;Zhou, Falin;Huang, Jianhua;Zhou, Falin
关键词：black tiger shrimp (
Penaeus monodon ); ammonia nitrogen stress; metabolic response; antioxidant enzymes; caspase -
The impact of microplastics on antibiotic resistance genes, metal resistance genes, and bacterial community in aquaculture environment
作者：Su, Haochang;Xu, Wujie;Hu, Xiaojuan;Xu, Yu;Wen, Guoliang;Cao, Yucheng;Su, Haochang;Xu, Wujie;Hu, Xiaojuan;Xu, Yu;Wen, Guoliang;Cao, Yucheng
关键词：Microplastics; Antibiotic resistance genes; Metal resistance genes; Bacterial community; Aquaculture
-
Integrated landscape of accessible regions and transcriptomic profiles in Penaeus monodon during ovarian maturation
作者：Li, Yundong;An, Jing;Jiang, Shigui;Jiang, Song;Huang, Jianhua;Yang, Lishi;Yang, Qibin;Shi, Jianzhi;Ma, Zhenhua;Zhou, Falin;Li, Yundong;Ma, Zhenhua;Zhou, Falin;Li, Yundong;Huang, Jianhua;Yang, Qibin;Shi, Jianzhi;Zhou, Falin;An, Jing
关键词：Shrimp; Ovary development; Eyestalk ablation; RNA-seq; Regulatory elements; ATAC-seq
-
Variation of Microorganisms and Water Quality, and Their Impacts on the Production of Penaeus vannamei in Small-Scale Greenhouse Ponds
作者：Wu, Siyu;Su, Haochang;Cao, Yucheng;Wen, Guoliang;Xu, Yu;Hu, Xiaojuan;Wu, Siyu;Wen, Guoliang;Shen, Bin;Hu, Xiaojuan;Su, Haochang;Cao, Yucheng;Wen, Guoliang;Xu, Yu;Hu, Xiaojuan;Su, Haochang;Cao, Yucheng;Wen, Guoliang;Xu, Yu;Hu, Xiaojuan;Su, Lei;Wu, Shanshan;Su, Yuting
关键词：small-scale greenhouse pond; microbial community; water quality; shrimp production; Penaeus vannamei
-
Cryopreservation Protocol Optimization for Penaeus monodon Sperm: Reagent Screening and Parameter Refinement
作者：Kong, Dewei;Jiang, Song;Shi, Jianzhi;Yang, Qibin;Huang, Jianhua;Li, Yundong;Ding, Yangyang;Wang, Jieyi;Qi, Xinyu;Zhou, Falin;Jiang, Song;Zhou, Falin;Liu, Tianmi
关键词：Penaeus monodon; cryopreservation; sperm; ultrastructure; cryoinjury mechanisms
-
Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of Penaeus semisulcatus
作者：Li, Yundong;Chen, Juan;Jiang, Song;Yang, Qibin;Yang, Lishi;Huang, Jianhua;Shi, Jianzhi;Ding, Yangyang;Zhou, Falin;Li, Yundong;Yang, Qibin;Zhou, Falin;Li, Yundong;Huang, Jianhua;Zhou, Falin;Jiang, Ziyi;Liu, Tianmi
关键词：Penaeus semisulcatus; amino acids; fatty acids; antioxidant enzyme activity; genetic diversity
