Genome-wide identification and comparative analysis of CLE family in rapeseed and its diploid progenitors
文献类型： 外文期刊
作者： Xie, Meili 1 ; Zhao, Chuanji 2 ; Song, Min 1 ; Xiang, Yang 1 ; Tong, Chaobo 2 ;
作者机构： 1.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang, Peoples R China
2.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Wuhan, Peoples R China
3.Qufu Normal Univ, Coll Life Sci, Qufu, Peoples R China
关键词： CLE peptide; Brassica napus; diploid progenitor; orthologous pairs; expression pattern; association mapping analysis; yield traits
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Crop genomics and breeding CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) proteins belong to a small peptide family in plants. During plant development, CLE gene family members play a pivotal role in regulating cell-to-cell communication and stem cell maintenance. However, the evolutionary process and functional importance of CLEs are unclear in Brassicaceae. In this study, a total of 70 BnCLEs were identified in Brassica napus (2n = 4x = 38, A(n)C(n)): 32 from the A(n) subgenome, 36 from the C-n subgenome, and 2 from the unanchored subgenome. Meanwhile, 29 BrCLE and 32 BoCLE genes were explored in Brassica rapa (2n = 2x = 20, A(r)) and Brassica oleracea (2n = 2x = 18, C-o). Phylogenetic analysis revealed that 163 CLEs derived from three Brassica species and Arabidopsis thaliana can be divided into seven subfamilies. Homology and synteny analyses indicated whole-genome triplication (WGT) and segmental duplication may be the major contributors to the expansion of CLE family. In addition, RNA-seq and qPCR analysis indicated that 19 and 16 BnCLEs were more highly expressed in immature seeds and roots than in other tissues. Some CLE gene pairs exhibited different expression patterns in the same tissue, which indicated possible functional divergence. Furthermore, genetic variations and regional association mapping analysis indicated that 12 BnCLEs were potential genes for regulating important agronomic traits. This study provided valuable information to understand the molecular evolution and biological function of CLEs in B. napus and its diploid progenitors, which will be helpful for genetic improvement of high-yield breeding in B. napus.
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