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High throughput oscillating-flow structure for polymerase chain reaction

文献类型： 外文期刊

作者： Zhou, Ranran 1 ; Sun, Yu 1 ; Luan, Yunxia 2 ; Jia, Wenshen 2 ; Yu, Yang 1 ; Yang, Jing 1 ; Chen, Jin 1 ; Wang, Fengchao 1 ;

作者机构： 1.Shanghai Inst Technol, Coll Sci, 100 Haiquan Rd, Shanghai 201418, Peoples R China

2.BAAFS, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China

期刊名称：AIP ADVANCES （ 影响因子：1.4； 五年影响因子：1.4 ）

ISSN：

年卷期： 2024 年 14 卷 6 期

页码：

收录情况： SCI

摘要： Polymerase chain reaction (PCR) is a revolutionary biomolecule technology that drives the development of bioscience. The current work reports a high throughput oscillating-flow PCR structure. In this chip, eight microchannels can simultaneously serve as PCR reaction chambers for nucleic acid sample amplification. To maximize the potential of this design, the effect of the chip structure parameter, fluid velocity, and channel spacing is simulated by finite element analysis. The thermal simulation results show that (1) when the distance between two temperature drivers is 4 mm, the width ratio is 1:3, the length of a single channel is 24 mm, the wall thickness is 3 mm (x) x 20 mm (y) x 1 mm (z), the diameter is 0.3 mm, and the fluid velocity is within 1 mm/s, two uniform temperature zones of 95 and 65 degrees C for the PCR process can be constructed and (2) the influence of channel spacing on temperature distribution is not significant. When the spacing is 2 mm, the relative standard deviations of the temperature for denaturation and the annealing/extension zone were 0.0706% and 0.0061%, respectively. Here, the chip sizes are 30 mm (x) x 54 mm (y) x 3 mm (z). (c) 2024 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND) license (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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