Aptamer functionalized magnetic hydrophobic polymer with synergetic effect for enhanced adsorption of alternariol from wheat
文献类型： 外文期刊
作者： Zhao, Liping 1 ; Li, Linsen 2 ; Liu, Zheng 3 ; Wang, Xinjie 1 ; Yang, Ruiqi 1 ; Luo, Zhaofeng 4 ; Fang, Xiaona 4 ; Luan, Yunxia 1 ;
作者机构： 1.Inst Qual Stand & Testing Technol BAAFS, Agr Prod Qual & Safety Risk Assessment Lab, Dept Agr, Beijing 100097, Peoples R China
2.Beijing Inst Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, Beijing, Peoples R China
3.Beijing Municipal Ctr Food Safety Monitoring & Ris, Beijing Inst Food Inspect & Res, Beijing 100085, Peoples R China
4.Chinese Acad Sci, Hangzhou Inst Med HIM, Aptamer Select Ctr, Key Lab Zhejiang Prov Aptamers & Theranost, Hangzhou 310022, Zhejiang, Peoples R China
关键词： Alternariol adsorption; Capture-SELEX; Aptamer; Magnetic hydrophobic polymer; Synergistic effect
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2024 年 435 卷
页码：
收录情况： SCI
摘要： A new adsorbent based on aptamer functionalized magnetic hydrophobic polymer (MHbPA) was developed for specific and efficient adsorption of alternariol (AOH). Through the synergistic effect of aptamer-AOH affinity and hydrophobic interaction of polymer, enhanced adsorption properties had been realized, in which AOH aptamer was the first selected through capture-SELEX with good specificity and affinity, and the targeting polymer was designed based on the hydrophobicity of AOH to increase the interaction. The proposed MHbPA demonstrated a high adsorption capacity of 187.6 ng/mg for AOH. The adsorption behavior was considered as Langmuir adsorption model and pseudo-secondary kinetic adsorption model. Notably, the adsorption of AOH in wheat powder samples could be accomplished within 10 mins with acceptable recoveries. The as designed adsorbent with synergistic effect provides new insights into the development of enhanced pretreatment materials for mycotoxin monitoring in complex food matrices with specific aptamer and targeting polymer.
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