文献类型： 外文期刊

作者： Yue, Junyang ¹ ; Chen, Qinyao ¹ ; Wang, Yingzhen ¹ ; Zhang, Lei ³ ; Ye, Chen ⁴ ; Wang, Xu ¹ ; Cao, Shuo ² ; Lin, Yunzhi ¹ ; Huang, Wei ⁶ ; Xian, He ⁷ ; Qin, Hongyan ⁸ ; Wang, Yanli ⁸ ; Zhang, Sijia ¹ ; Wu, Ying ¹ ; Wang, Songhu ¹ ; Yue, Yi ¹ ; Liu, Yongsheng ¹ ;

作者机构： 1.Anhui Agr Univ, Sch Hort, Hefei 230036, Anhui, Peoples R China

2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518124, Guangdong, Peoples R China

3.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430064, Hubei, Peoples R China

4.Anhui Agr Univ, Sch Informat & Comp, Hefei 230036, Anhui, Peoples R China

5.Sichuan Univ, Coll Life Sci, Minist Educ Key Lab Bio resource & Eco Environm, State Key Lab Hydraul & Mt River Engn, Chengdu 610064, Sichuan, Peoples R China

6.Anhui Double Helix Gene Technol Corp, Dept Bioinformat, Hefei 230022, Anhui, Peoples R China

7.Xinjiang Acad Agr Sci, Comprehens Testing Ground, Urumqi 830012, Xinjiang, Peoples R China

8.Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, Changchun 130112, Jilin, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 2022影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2023 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Kiwifruit is an economically and nutritionally important fruit crop with extremely high contents of vitamin C. However, the previously released versions of kiwifruit genomes all have a mass of unanchored or missing regions. Here, we report a highly continuous and completely gap-free reference genome of Actinidia chinensis cv. 'Hongyang', named Hongyang v4.0, which is the first to achieve two de novo haploid-resolved haplotypes, HY4P and HY4A. HY4P and HY4A have a total length of 606.1 and 599.6 Mb, respectively, with almost the entire telomeres and centromeres assembled in each haplotype. In comparison with Hongyang v3.0, the integrity and contiguity of Hongyang v4.0 is markedly improved by filling all unclosed gaps and correcting some misoriented regions, resulting in similar to 38.6-39.5 Mb extra sequences, which might affect 4263 and 4244 protein-coding genes in HY4P and HY4A, respectively. Furthermore, our gap-free genome assembly provides the first clue for inspecting the structure and function of centromeres. Globally, centromeric regions are characterized by higher-order repeats that mainly consist of a 153-bp conserved centromere-specific monomer (Ach-CEN153) with different copy numbers among chromosomes. Functional enrichment analysis of the genes located within centromeric regions demonstrates that chromosome centromeres may not only play physical roles for linking a pair of sister chromatids, but also have genetic features for participation in the regulation of cell division. The availability of the telomere-to-telomere and gap-free Hongyang v4.0 reference genome lays a solid foundation not only for illustrating genome structure and functional genomics studies but also for facilitating kiwifruit breeding and improvement.