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MbWRKY53, a M. baccata WRKY Transcription Factor, Contributes to Cold and Drought Stress Tolerance in Transgenic Arabidopsis thaliana

文献类型： 外文期刊

作者： Liu, Wanda 1 ; Wang, Tianhe 1 ; Liang, Xiaoqi 2 ; Ye, Qinglei 3 ; Wang, Yu 1 ; Han, Jilong 1 ; Han, Deguo 2 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Hort Branch, Harbin 150069, Peoples R China

2.Northeast Agr Univ, Key Lab Biol & Genet Improvement Hort Crops Northe, Minist Agr & Rural Affairs, Natl Local Joint Engn Res Ctr Dev & Utilizat Small, Harbin 150038, Peoples R China

3.Heilongjiang Agr Technol Extens Stn, Harbin 150090, Peoples R China

关键词： Malus baccata (L.) Borkh; WRKY transcription factor; low temperature stress; drought stress

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 14 期

页码：

收录情况： SCI

摘要： Apple is an important horticultural crop, but various adverse environmental factors can threaten the quality and yield of its fruits. The ability of apples to resist stress mainly depends on the rootstock. Malus baccata (L.) Borkh. is a commonly used rootstock in Northeast China. In this study, it was used as the experimental material, and the target gene MbWRKY53 was screened through transcriptome analysis and Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) after cold and drought treatment. Bioinformatics analysis revealed that this transcription factor (TF) belonged to the WRKY TF family, and its encoded protein was localized in the nucleus. RT-qPCR showed that the gene was more easily expressed in roots and young leaves and is more responsive to cold and drought stimuli. Functional validation in Arabidopsis thaliana confirmed that MbWRKY53 can enhance plant tolerance to cold and drought stress. Furthermore, by analyzing the expression levels of genes related to cold and drought stress in transgenic Arabidopsis lines, it was inferred that this gene can regulate the expression of stress-related genes through multiple pathways such as the CBF pathway, SOS pathway, Pro synthesis pathway, and ABA-dependent pathways, enhancing the adaptability of transgenic Arabidopsis to cold and drought environments.

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