Ecytonucleospora hepatopenaei (EHP) disease prevalence and mortality in Litopenaeus vannamei: a comparative study from Eastern India shrimp farms
文献类型： 外文期刊
作者： Kumar, Vikash 1 ; Das, Basanta Kumar 2 ; Dhar, Souvik 1 ; Bisai, Kampan 1 ; Pande, Gde Sasmita Julyantoro 3 ; Zheng, Xiaoting 4 ; Parida, Satya Narayan 1 ; Adhikari, Anupam 1 ; Jana, Asim Kumar 1 ;
作者机构： 1.ICAR Cent Inland Fisheries Res Inst CIFRI, Aquat Environm Biotechnol AEB Div, Barakpur 700120, India
2.Cent Inland Fisheries Res Inst CIFRI, ICAR, Barakpur 700120, India
3.Udayana Univ, Fac Marine Sci & Fisheries, Dept Aquat Resource Management, Denpasar 80361, Kuta Selatan, Indonesia
4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
关键词：
EHP;
期刊名称：BMC MICROBIOLOGY （ 影响因子：4.2； 五年影响因子：4.8 ）
ISSN： 1471-2180
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： Ecytonucleospora hepatopenaei (EHP), a microsporidian parasite first named and characterized from the Penaeus monodon (black or giant tiger shrimp), causes growth retardation and poses a significant threat to shrimp farming. We observed shrimp farms associated with disease conditions during our fish disease surveillance and health management program in West Bengal, India. Shrimp exhibited growth retardation and increased size variability, particularly in advanced stages, exhibiting soft shells, lethargy, reduced feeding and empty midguts. Floating white feces were observed on the surface of the pond water. Suspecting a microbial infection, the shrimp samples were collected and aseptically brought to the ICAR-CIFRI laboratory for molecular confirmation. A nested PCR was used to screen shrimp tissue, feces, feed and environmental samples for the possible presence of hepatopancreatic microsporidiosis caused by Ecytonucleospora hepatopenaei. The results confirmed that the shrimp samples were positive for EHP. Histopathological investigation revealed mature spores in the HP tubule lumen and epithelial cells along with necrotic tubule in the symptomatic group. Further, the transcription analysis revealed that ProPO, Hsp70 and alpha 2-macroglobulin genes were significantly upregulated, while decreased expression of LGBP, PXN and Integrin ss was observed in shrimp infected with Hepatopancreatic microsporidiosis. Furthermore, compared with the healthy group, significant intestinal bacteria changes were observed in the EHP-infected group. The in vivo survival assay, using crustacean animal model Artemia franciscana, suggests that symptomatic shrimp gut samples harbour pathogenic Vibrio parahaemolyticus, V. harveyi and V. campbellii. These results significantly advance our understanding of the molecular and ecological aspects of EHP pathobiology.
- 相关文献
作者其他论文 更多>>
-
Anti-Vibrio potential of essential oil components and the role of cinnamaldehyde in Pacific oyster (Magallana gigas) juveniles
作者：Loor, Alfredo;Perelli, Joshua;Bossier, Peter;Loor, Alfredo;Perelli, Joshua;Bossier, Peter;Loor, Alfredo;Zheng, Xiaoting
关键词：Magallana gigas; Cinnamaldehyde; Vibrio infection; Immune response; Antioxidant; TRP channels
-
The Path Forward for China's Bullfrog Industry: Exploring Green Farming Models
作者：Zheng, Xiaoting;Zhang, Jiasong;Zheng, Xiaoting;Yang, Jinlong;Declercq, Annelies M.;Zhang, Jiasong
关键词：Aquarana catesbeiana; bullfrog industry; China; farming models; farming practice; recirculating aquaculture systems
-
The effects of citral on the intestinal health and growth performance of American bullfrogs (Aquarana catesbeiana)
作者：Zheng, Xiaoting;Chen, Qiuyu;Liang, Xueying;Xie, Jingyi;Dong, Hongbiao;Zhang, Jiasong;Zheng, Xiaoting;Yang, Jinlong;Loor, Alfredo;Dong, Hongbiao;Zhang, Jiasong
关键词：Bullfrog; Citral; Growth performance; Intestinal health; Oxidative stress
-
Assessment of the molecular mechanism in fish using eugenol as anesthesia based on network pharmacology
作者：Zeng, Xiangbing;Zheng, Xiaoting;Dong, Hongbiao;Zhang, Jiasong;Zeng, Xiangbing;Zheng, Xiaoting;Dong, Hongbiao;Zhang, Jiasong;Wu, Jingru
关键词：Eugenol; Anesthesia; Fish; Network pharmacology; Molecular docking
-
Effects of nitrite exposure on biochemical parameters and liver histopathology in American bullfrogs (Aquarana catesbeiana)
作者：Chen, Qiuyu;Huang, Jianhua;Zhang, Jiasong;Chen, Qiuyu;Zheng, Xiaoting;Liang, Xueying;Dong, Hongbiao;Huang, Jianhua;Zhang, Jiasong;Chen, Qiuyu;Zheng, Xiaoting;Liang, Xueying;Dong, Hongbiao;Zhang, Jiasong;Kumar, Vikash;Huang, Jianhua
关键词：Amphibians; Nitrite; Hepatotoxicity; Histopathology; Oxidative stress
-
In vitro and in vivo characterization of citral and limonene combinations against Vibrio campbellii in brine shrimp
作者：Zheng, Xiaoting;Zheng, Xiaoting;Declercq, Annelies M.;Bossier, Peter;Vanrompay, Daisy;Feyaerts, Adam F.;Van Dijck, Patrick
关键词：Citral; Limonene; Vibrio campbellii; Brine shrimp; Essential oil component
-
The Composition and Function of Intestinal Microbiota Were Altered in Farmed Bullfrog Tadpoles (Aquarana catesbeiana) during Metamorphosis
作者：Zheng, Xiaoting;Chen, Qiuyu;Liang, Xueying;Dong, Hongbiao;Liu, Chang;Zhang, Jiasong;Zheng, Xiaoting;Yang, Jinlong;Kumar, Vikash;Loor, Alfredo;Dong, Hongbiao;Zhang, Jiasong
关键词：
Aquarana catesbeiana ; tadpole; intestinal microbiota; metamorphosis
