A self-assembled 3D nanoflowers based nano-ELISA platform for the sensitive detection of pyridaben
文献类型： 外文期刊
作者： Chen, He 1 ; An, Li 1 ; Li, Meng 1 ; Liu, Hao 3 ; Jin, Zhong 1 ; Ma, Huan 1 ; Ma, Jingwei 1 ; Zhou, Juan 1 ; Duan, Ran 1 ; Zhang, Di 1 ; Cao, Xiu 1 ; Wang, Tieliang 1 ; Wu, Xujin 1 ;
作者机构： 1.Henan Acad Agr Sci, Inst Qual & Safety Agroprod, Zhengzhou 450002, Peoples R China
2.Key Lab Grain Qual & Safety & Testing Henan Prov, Zhengzhou 450002, Peoples R China
3.Henan Univ, Sch Life Sci, Kaifeng 475004, Henan, Peoples R China
4.Henan Inst Sci & Technol, Sch Resources & Environm, Xinxiang 453003, Henan, Peoples R China
关键词： Protein -inorganic nanoflower; Hierarchical structure; Protein immobilization; nano-ELISA; Pesticide residue
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2024 年 445 卷
页码：
收录情况： SCI
摘要： Biomimetic methods are invariably employed to synthesize hybrid organic-inorganic multilevel structure nanoflowers with self-assembly processes in aqueous solutions, which is an ideal way to meet the challenges of immobilizing antibodies or enzymes in nanomaterial based enzyme-linked immunosorbent assay (nano-ELISA). In this study, we developed protein-inorganic hybrid 3D nanoflowers composed of bovine serum albumin (BSA), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (IgG-HRP) and copper(II) phosphate (BSA-(IgGHRP)-Cu3(PO4)2) using a self-assembly biomimetic method. The preparation process avoided the use of any organic solvent and protein immobilization did not require covalent modifications. Additionally, the unique hierarchical structure enhances the thermal and storage stability of HRP. The BSA-(IgG-HRP)-Cu3(PO4)2 hybrid 3D nanoflower was then applied to a nano-ELISA platform for pyridaben detection, achieving a 50% inhibition concentration of 3.90 ng mL-1. The nano-ELISA achieved excellent accuracy for pyridaben detection. Such a novel BSA-(IgG-HRP)-Cu3(PO4)2 hybrid 3D nanoflower provide an excellent reagent for small molecule immunoassay.
- 相关文献
作者其他论文 更多>>
-
Advances in aggregation-induced emission fluorescent probes for reactive sulfur species detection
作者：Zhang, Qianqian;Zhang, Zezhi;Liu, Jin;Song, Ajuan;Li, Chengwei;Yang, Xiaopeng;Zhang, Di;Ye, Yong
关键词：Fluorescent probe; Aggregation-induced emission; Reactive Sulfur species; Detection
-
A naphthimide based fluorescent probe for the detection of thiophenols and its application in actual water and food
作者：Zhang, Di;Duan, Ran;Zhou, Xiaohua;Shi, Jiacheng;Chen, He;Guo, Jie;Li, Man;Wang, Tieliang;Wu, Xujin;Ma, Zhiwei;Shi, Jiacheng
关键词：Thiophenol; Fluorescence probe; Off-on; Water; Food
-
Quantitative tracing of typical herbicides and their metabolites in sorghum agrosystems for fate tendency and cumulative risk
作者：Li, Tong;Zhao, Lilin;Chen, Zenglong;Li, Tong;Cheng, Youpu;Wu, Xujin;Zheng, Lufei
关键词：UHPLC-MS/MS; Herbicides; Metabolites; Combined exposure; Risk assessment
-
Resistance risk and mechanism of prochloraz in Fusarium solani
作者：Mao, Xuewei;Cao, Tangbo;Chen, Liting;Li, Min;Zhao, Xingchen;Liu, Hao;Wang, Li;Zhou, Lin;Mao, Xuewei;Wang, Li;Zhou, Lin;Mao, Xuewei;Wang, Li;Zhou, Lin;Mao, Xuewei;Duan, Yun
关键词：Fusarium solani; Prochloraz; Baseline sensitivity; Resistance mechanisms; Resistance management
-
Efficient inactivation of antibiotic resistant bacteria by iron-modified biochar and persulfate system: Potential for controlling antimicrobial resistance spread and mechanism insights
作者：Duan, Ran;Ma, Shuanglong;Ma, Yanbing;Li, Guangxin;Fu, Haichao;Zhao, Peng;Duan, Ran;Wu, Xujin;Xu, Shengjun;Du, Jinge
关键词：Iron-modified biochar; Persulfate-based advanced oxidation processes; Antibiotic resistant bacteria inactivation; Inhibition mechanism
-
Cancer-targeted and biomarker-activated fluorescent probe for diagnosis imaging and therapy
作者：Liu, Jianfei;Sun, Junmin;Zhao, Yufen;Ye, Yong;Liu, Jianfei;Sun, Junmin;Zhao, Yufen;Ye, Yong;Yang, Xiaopeng;Zhang, Di
关键词：Multi-functional; Fluorescent probe; Cancer diagnosis and therapy; Ferroptosis
-
MgO-embedded S-doped porous biochar composites for efficient removal Cd(II) and Pb(II) in water: DFT studies and mechanistic insights
作者：Hao, Peixin;Fu, Haichao;Ma, Shuanglong;Li, Guangxin;Sui, Fuqing;Li, Chang;Zhao, Peng;Hao, Peixin;Fu, Haichao;Ma, Shuanglong;Li, Guangxin;Sui, Fuqing;Li, Chang;Zhao, Peng;Xue, Weijie;Xiong, Shiwu;Duan, Ran
关键词：Sodium thiosulfate; Magnesium oxide; Cotton straw biochar; Heavy metals; Density functional theory
