文献类型： 外文期刊
作者： Kong, Xuewei 1 ; Wang, Hui 1 ; Guo, Genglin 1 ; Li, Pei 1 ; Tong, Panpan 4 ; Liu, Maojun 5 ; Ma, Xun 6 ; Dong, Chen 7 ; Li, Yubao 8 ; Zhang, Haiyan 9 ; Zhang, Wei 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China
2.Minist Agr, Key Lab Anim Bacteriol, Nanjing, Peoples R China
3.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing, Peoples R China
4.Shihezi Agr Univ, Coll Vet Med, Shihezi, Xinjiang, Peoples R China
5.Jiangsu Acad Agr Sci, Nanjing, Peoples R China
6.Xinjiang Agr Univ, Coll Anim Sci & Technol, Urumqi, Xinjiang, Peoples R China
7.Jiangsu Prov Cdc Jiangsu Prov Ctr Dis Control & P, Nanjing, Peoples R China
8.Liaocheng Univ, Agr Sci & Engn Sch, Liaocheng, Shandong, Peoples R China
9.Wuhu Inst Technol, Dept Food & Biol Engn, Wuhu, Peoples R China
关键词： Bacteriophage; Phage therapy; STEC; APEC; Pan-genome
期刊名称：VIRUS GENES （ 影响因子：2.198； 五年影响因子：1.938 ）
ISSN： 0920-8569
年卷期：
页码：
收录情况： SCI
摘要： Multiple pathogenic types or serotypes restrict treatment for colibacillosis. In addition, rising antibiotic resistance has heightened public awareness to prevent and control pathogenic Escherichia coli. The bacteriophage is a viable technique to treat colibacillosis as an alternative to antibiotics. P762, a coliphage isolated from duck farm sewage, was demonstrated to cloud lyse Shiga toxin-producing Escherichia Coli serotypes O157 and non-O157 (17/39), Avian pathogenic E. coli covered serotype O78, O83, and O9 (5/19), and other pathogenic Escherichia coli (5/17). Additional fundamental biological characteristics analysis revealed that P762 is stable at pH 3 similar to 11 and temperature between 4 degrees C and 60 degrees C, and its optimum multiplicity of infection (MOI) is 0.1. The one-step curve of P762 exhibited three bursts of growth stage: two rapid and one slow stage. Furthermore, the first rapid burst size is 80 CFU/PFU, the burst size of the slow stage is 10 CFU/PFU, and the second rapid burst size is about 990 CFU/PFU. In addition, P762 can form a "halo" on a double agar plate, implying that the phage secretes depolymerase. With 95.14% identity and 90% query coverage, genome sequence analysis revealed that P762 is most closely related to Escherichia phage DY1, which belongs to the genus Kayfunavirus. After screening using RAST and VFDB, no virulence factors were discovered in P762. In vitro antibacterial tests revealed that P762 has high bactericidal activity in lettuce leaves contaminated with STEC. In conclusion, phage P762 might be employed in the future to prevent and control pathogenic Escherichia coli.
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