Preventive Effects of Anthocyanins from Lycium ruthenicum Murray in High-Fat Diet-Induced Obese Mice Are Related to the Regulation of Intestinal Microbiota and Inhibition of Pancreatic Lipase Activity
文献类型： 外文期刊
作者： Li, Na 1 ; Liu, Xi 1 ; Zhang, Jing 1 ; Lang, Yan-Zhi 1 ; Lu, Lu 2 ; Mi, Jia 2 ; Cao, You-Long 2 ; Yan, Ya-Mei 2 ; Ran, Lin-Wu 1 ;
作者机构： 1.Ningxia Med Univ, Sch Publ Hlth & Management, Key Lab Environm Factors & Chron Dis Control, Yinchuan 750004, Ningxia, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Goji Berry Res Inst, Yinchuan 750002, Ningxia, Peoples R China
3.Ningxia Med Univ, Lab Anim Ctr, Yinchuan 750004, Ningxia, Peoples R China
关键词： L; ruthenicum; obesity; intestinal microbiota; pancreatic lipase
期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）
ISSN：
年卷期： 2022 年 27 卷 7 期
页码：
收录情况： SCI
摘要： Lyciumruthenicum Murray (L. ruthenicum) has been used both as traditional Chinese medicine and food. Recent studies indicated that anthocyanins are the most abundant bioactive compounds in the L. ruthenicum fruits. The purpose of this study was to investigate the preventive effects and the mechanism of the anthocycanins from the fruit of L. ruthenicum (ACN) in high-fat diet-induced obese mice. In total, 24 male C57BL/6J mice were divided into three groups: control group (fed a normal diet), high-fat diet group (fed a high-fat diet, HFD), and HFD +ACN group (fed a high-fat diet and drinking distilled water that contained 0.8% crude extract of ACN). The results showed that ACN could significantly reduce the body weight, inhibit lipid accumulation in liver and white adipose tissue, and lower the serum total cholesterol and low-density lipoprotein cholesterol levels compared to that of mice fed a high-fat diet. 16S rRNA gene sequencing of bacterial DNA demonstrated that ACN prevent obesity by enhancing the diversity of cecal bacterial communities, lowering the Firmicutes-to-Bacteroidota ratio, increasing the genera Akkermansia, and decreasing the genera Faecalibaculum. We also studied the inhibitory effect of ACN on pancreatic lipase. The results showed that ACN has a high affinity for pancreatic lipase and inhibits the activity of pancreatic lipase, with IC50 values of 1.80 (main compound anthocyanin) and 3.03 mg/mL (crude extract), in a competitive way. Furthermore, fluorescence spectroscopy studies showed that ACN can quench the intrinsic fluorescence of pancreatic lipase via a static mechanism. Taken together, these findings suggest that the anthocyanins from L. ruthenicum fruits could have preventive effects in high-fat-diet induced obese mice by regulating the intestinal microbiota and inhibiting the pancreatic lipase activity.
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