Star1 gene mutation reveals the essentiality of 11-ketotestosterone and glucocorticoids for male fertility in Nile Tilapia (Oreochromis niloticus)
文献类型： 外文期刊
作者： Su, Yun 1 ; Wu, You 2 ; Ye, Maolin 2 ; Zhao, Chenhua 2 ; Li, Lu 2 ; Cai, Jing 2 ; Chakraborty, Tapas 3 ; Yang, Lanying 2 ; Wang, Deshou 2 ; Zhou, Linyan 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Fisheries Engn Inst, Beijing, Peoples R China
2.Southwest Univ, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing, Minist Educ,Sch Life Sci, Chongqing 400715, Peoples R China
3.Kyushu Univ, Fac Agr, Fukuoka 8190395, Japan
关键词： Steroid biosynthesis; Star genes; 11-KT; Glucocorticoids; Male fertility
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：1.8； 五年影响因子：2.1 ）
ISSN： 1096-4959
年卷期： 2024 年 273 卷
页码：
收录情况： SCI
摘要： Steroidogenic acute regulatory protein (Star) plays an essential role in the biosynthesis of corticosteroids and sex steroids by mediating the transport of cholesterol from the outer to the inner membrane of mitochondria. Two duplicated Star genes, namely star1 and star2, have been identified in non-mammalian vertebrates. To investigate the roles of star genes in fish steriodogenesis, we generated two mutation lines of star1(-/-) and star1(-/-)/star2(-/- )in Nile tilapia (Oreochromis niloticus). Previous studies revealed that deficiency of star2 gene caused delayed spermatogenesis, sperm apoptosis and sterility in male tilapia. Our present data revealed that mutation of star genes impaired male fertility. Disordered seminiferous lobules and spermatic duct obstruction were found in the testis of both types of mutants. Moreover, significant decline in semen volume, sperm abnormality and impaired fertility were also detected in star1(-/-) and star1(-/-) /star2(-/-) males. In star1(-/-) male fish, lipid accumulation, upregulation of steroidogenic enzymes, and significant decline of androgens were found. Additionally, hyperplasic interrenal cells, elevated steroidogenic gene expression level and decline of serum glucocorticoids were detected in star1 mutants. Intriguingly, either 11-KT or cortisol supplementation successfully rescued the impaired fertility of the star1(-/-) mutants. Taken together, these results further indicate that Star1 might play critical roles in the production of both 11-KT and glucocorticoids, which are indispensable for the maintenance of male fertility in fish.
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