Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda
文献类型： 外文期刊
作者： Yao, Yang 1 ; Lin, Dong-Jiang 1 ; Cai, Xiang-Yun 2 ; Wang, Ran 3 ; Hou, You-Ming 2 ; Hu, Chao-Hua 1 ; Gao, San-Ji 1 ; Wang, Jin-Da 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Plant Protect, State KeyLaboratory Ecol Pest Control Fujian & Tai, Minist Educ, Fuzhou, Peoples R China
3.Inst Plant Protect, Beijing Acad Agr & Forestry, Beijing, Peoples R China
关键词： RNA inference; dsRNase; efficiency; liposome; Spodoptera frugiperda
期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.755； 五年影响因子：5.316 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency. In the current study, we identified four genes coding for dsRNases from the Spodoptera frugiperda genome. Spatial and temporal expression analysis showed that those dsRNases were highly expressed in the midgut and old larvae. Then a delivery method was applied for inducing efficient RNAi based on dsRNA encapsulated by liposome. Furthermore, we assessed degradation efficiency by incubation with dsRNA with gut juice or hemocoel to characterize potential roles of different SfdsRNases after suppression of SfdsRNase. The result showed that interferenced with any sfdsRNase reduced the degradation of exogenous dsRNA in midgut, interfered with sfdsRNase1 and sfdsRNase3 slowed down the degradation of exogenous dsRNA in hemolymph. Our data suggest the evolutionary expansion and multiple high activity dsRNase genes would take part in the RNAi obstinate in S. frugiperda, besides we also provide an efficient RNAi method for better use of RNAi in S. frugiperda.
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