Anti-Vibrio Response of CarcininPm 1 from Penaeus monodon and Its Heterologous Expression
文献类型： 外文期刊
作者： Zhou, Liang 1 ; Li, Guoqiang 1 ; Li, Anguo 1 ; Jiao, Yang 1 ; Huang, Jianhua 2 ; Wang, Chaogang 1 ;
作者机构： 1.Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Technol Res Ctr Marine Algal Bioengn, Shenzhen 518060, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 518121, Guangdong, Peoples R China
期刊名称：2018 INTERNATIONAL SEMINAR ON FOOD SAFETY AND ENVIRONMENTAL ENGINEERING (FSEE 2018)
ISSN： 2267-1242
年卷期： 2019 年 78 卷
页码：
收录情况： SCI
摘要：
Crustins are crucial antimicrobial peptides in shrimp and play very important roles in innate immunity. In this research, a Type I crustin from Penaeus monodon (CarcininPm1) contained 108 residues was studied. The first 16 residues are signal peptide. It contained ten cysteines but did not form an intact whey acidic protein (WAP) domain. CarcininPm1 was observed to widely distribute in all tissues, while highly expressed in intestine. The expression level of CarcininPm1 in hepatopancreas was up-regulated 1220 times during 4-12h post challenged by Vibrio parahaemolyticus. And the transcription in heart, stomach and gills was also significantly enhanced at 4h post challenge. The mature peptide was expressed successfully in Eschericha coli by fusing to a SUMO protein, with protein production around 8 mg/mL. After cleavage with SUMO protease, carcininPm1 was obtained indicating its potential applications.
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