Simultaneous One-Step Preparation of β/κ-Carrapentaose and 3,6-Anhydro-D-galactose by Cascading κ-Carrageenase and an Exo-α-3,6-Anhydro-D-galactosidase
文献类型： 外文期刊
作者： Jiang, Chengcheng 1 ; Wang, Wei 1 ; Sun, Jingjing 1 ; Hao, Jianhua 1 ; Mao, Xiangzhao 4 ;
作者机构： 1.Chinese Acad Fishery Sci, Lab Marine Drugs & Byproducts, Natl Lab Marine Sci & Technol, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
3.Jiangsu Collaborat Innovat Ctr Exploitat & Utiliza, Lianyungang 222005, Peoples R China
4.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
关键词： carrageenolytic enzymes; carrageenan; beta/kappa-carrapentaose; 3; 6-anhydro-D-galactose; cascade system
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 47 期
页码：
收录情况： SCI
摘要： Carrageenan oligosaccharides have shown promising bioavailability and possess a variety of physiological activities, making them highly suitable for use in the food, pharmaceutical, and agricultural industries. The preferred method for producing carrageenan oligosaccharides is using various carrageenolytic enzymes, as it offers mild reaction conditions, high efficiency, and product specificity. However, there is still a lack of specific applications for using these enzymes to prepare odd-numbered carrageenan-oligosaccharides (OCOSs). Our previous research identified a more convenient route for simultaneously preparing OCOSs and 3,6-anhydro-D-galactose (D-AHG) using only two types of carrageenolytic enzymes: kappa-carrageenase and exo-alpha-3,6-anhydro-D-galactosidase (D-ADAGase). In this study, we utilized a CipA-based self-assembly system to cascade kappa-carrageenase CaKC16A and D-ADAGase ZuGH129A for one-step preparation of beta/kappa-carrapentaose, G-(DA-G4S)2, and D-AHG from degrading beta/kappa-carrageenan. This self-assembled enzyme, namely CipA-CaKC16A-ZuGH129A, can be easily obtained through a simple centrifugation process. The final optimized enzymatic process produced 0.74 g/L G-(DA-G4S)2 and 0.13 g/L D-AHG. This cascade system of different types of carrageenolytic enzymes has the potential to achieve the preparation of various types of carrageenan oligosaccharides.
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