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Identification and Expression Analysis of miR160 and Their Target Genes in Cucumber

文献类型： 外文期刊

作者： Li, Yaoyao 1 ; Luo, Weirong 1 ; Sun, Yongdong 1 ; Chang, Huaicheng 1 ; Ma, Kai 3 ; Zhao, Zhenxiang 1 ; Lu, Lin 1 ;

作者机构： 1.Henan Inst Sci & Technol, Sch Hort & Landscape Architecture, Xinxiang 453003, Henan, Peoples R China

2.Hort Plant Resource Utilizat & Germplasm Enhancem, Henan Prov Engn Res Ctr, Xinxiang 453003, Henan, Peoples R China

3.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China

关键词： Cucumber (Cucumis sativus L; ); Fruit expansion; miR160; Auxin response factor (ARF); Gene structure; qRT-PCR

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）

ISSN： 0006-2928

年卷期：

页码：

收录情况： SCI

摘要： miR160 plays a crucial role in various biological processes by regulating their target gene auxin response factor (ARF) in plants. However, little is known about miR160 and ARF in cucumber fruit expansion. Here, 4 Csa-MIR160 family members and 17 CsARFs were identified through a genome-wide search. Csa-miR160 showed a closer relationship with those in melon. Phylogenetic analysis revealed that CsARFs were divided into four classes and most of CsARFs presented a closer evolutionary relationship with those from tomato. Putative cis-elements analysis predicted that Csa-MIR160 and CsARFs were involved in light, phytohormone and stress response, which proved that they might take part in light, phytohormone and stress signaling pathway by the miR160-ARF module. In addition, CsARF5, CsARF11, CsARF13 and CsARF14 were predicted as the target genes of Csa-miR160. qRT-PCR revealed that Csa-miR160 and their target gene CsARFs were differentially expressed in differential cucumber tissues and developmental stages. Csa-miR160d was only expressed in the expanded cucumber fruit. CsARF5, CsARF11 and CsARF13 exhibited the lower expression in the expanded fruit than those in the ovary, while, CsARF14 showed the reverse trend. Our results suggested that Csa-miR160d might play a crucial role in cucumber fruit expansion by negatively targeting CsARF5, CsARF11 and CsARF13. This is the first genome-wide analysis of miR160 in cucumber. These findings provide useful information and resources for further studying the role of miR160 and ARF in cucumber fruit expansion.

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