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Construction of a genetic linkage map in Fenneropenaeus chinensis (Osbeck) using RAPD and SSR markers

文献类型： 外文期刊

作者： Sun, Zhaoning 1 ; Liu, Ping 1 ; Li, Jian 1 ; Meng, Xianhong 1 ; Zhang, Xiumei 3 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Beijing 100039, Peoples R China

3.Ocean Univ China, Div Life Sci & Technol, Qingdao 266003, Peoples R China

期刊名称：HYDROBIOLOGIA （ 影响因子：2.694； 五年影响因子：2.781 ）

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收录情况： SCI

摘要： The primary genetic linkage maps of Fenneropenaeus chinensis (Osbeck) were constructed by using the two-way pseudo-testcross strategy with RAPD and SSR markers. Parents and F1 progeny were used as segregating populations. Sixty-one RAPD primers and 20 pairs of SSR primers were screened from 460 RAPD primers and 44 pairs of SSR primers. These primers were used to analyze the parents and 82 progeny of the mapping family. About 146 primers (128 RAPDs, 18 microsatellites) in the female and 127 primers (109 RAPDs, 18 microsatellites) in the male were segregating markers. The female linkage map included eight linkage groups, nine triplets and 14 doublets, spanning 1,173 cM with the average marker density of 11.28 cM, and the observed coverage was 59.36%. The male linkage map included 10 linkage groups, 12 triplets and seven doublets, spanning 1,144.6 cM with the average marker density of 12.05 cM, and the observed coverage was 62.01%. The construction of the F. chinensis genetic linkage maps here opened a new prospect for marker-assisted selection program, comparative genomics and quantitative trait loci (QTL) gene location and cloning.

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