Fine Mapping and Characterization of a Major Gene Responsible for Chlorophyll Biosynthesis in Brassica napus L.
文献类型： 外文期刊
作者： Pang, Chengke 1 ; Zhang, Wei 2 ; Peng, Menlu 1 ; Zhao, Xiaozhen 1 ; Shi, Rui 1 ; Wu, Xu 2 ; Chen, Feng 2 ; Sun, Chengming 2 ; Wang, Xiaodong 2 ; Zhang, Jiefu 1 ;
作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Ind Crops, Key Lab Cotton & Rapeseed, Minist Agr & Rural Afairs, Nanjing 210014, Peoples R China
3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
关键词： Brassica napus; quantitative trait loci sequencing; candidate gene; chlorophyll deficiency; fine mapping; molecular marker
期刊名称：BIOMOLECULES （ 影响因子：6.064； 五年影响因子：6.191 ）
ISSN：
年卷期： 2022 年 12 卷 3 期
页码：
收录情况： SCI
摘要： Rapeseed (Brassica napus L.) is mainly used for oil production and industrial purposes. A high photosynthetic efficiency is the premise of a high yield capable of meeting people's various demands. Chlorophyll-deficient mutants are ideal materials for studying chlorophyll biosynthesis and photosynthesis. In a previous study, we obtained the mutant yl1 for leaf yellowing throughout the growth period by ethyl methanesulfonate mutagenesis of B. napus. A genetic analysis showed that the yl1 chlorophyll-deficient phenotype was controlled by one incompletely dominant gene, which was mapped on chromosome A03 by a quantitative trait loci sequencing analysis and designated as BnA03.Chd in this study. We constructed an F-2 population containing 5256 individuals to clone BnA03.Chd. Finally, BnA03.Chd was fine-mapped to a 304.7 kb interval of the B. napus 'ZS11' genome containing 58 annotated genes. Functional annotation, transcriptome, and sequence variation analyses confirmed that BnaA03g0054400ZS, a homolog of AT5G13630, was the most likely candidate gene. BnaA03g0054400ZS encodes the H subunit of Mg-chelatase. A sequence analysis revealed a single-nucleotide polymorphism (SNP), causing an amino-acid substitution from glutamic acid to lysine (Glu1349Lys). In addition, the molecular marker BnaYL1 was developed based on the SNP of BnA03.Chd, which perfectly cosegregated with the chlorophyll-deficient phenotype in two different F-2 populations. Our results provide insight into the molecular mechanism underlying chlorophyll synthesis in B. napus.
- 相关文献
作者其他论文 更多>>
-
Hatcheries' contamination: the molecular characterization and pathogenicity of Mycoplasma gallisepticum isolates in China
作者：Fang, Huanxin;Li, Qi;Li, Pei;Xu, Sixiang;Tan, Libin;Zhu, Xihui;Zheng, Xiangkuan;Tian, Rui;Qi, Zitai;Li, Chenxi;Yu, Yanfei;Zhang, Wei;Zhang, Chunyan;Qi, Zitai;Yu, Yanfei
关键词：Mycoplasma gallisepticum; Hatcheries' contamination; Pipping failure embryos; China I Clade; Vertical and horizontal transmission
-
Knocking out artificially selected gene GmAOC4H8 improves germination in soybean
作者：Zhang, Wei;Li, Songsong;Xu, Wenjing;Wang, Qiong;Zhang, Hongmei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Chen, Huatao;Xu, Donghe
关键词：
-
FabG moonlights as an extracellular adhesin mediates cytoadhesion of Streptococcus suis via interaction with plasminogen
作者：Guo, Genglin;Zhou, Yu;Li, Pei;Yu, Yanfei;Zhang, Wei;Zhou, Yu;Li, Pei;Zhang, Wei;Li, Quan;Yu, Yanfei
关键词：Streptococcus suis; Adhesin; FabG; Plasminogen; Extracellular matrix; Complement
-
Optimized straw incorporation depth can improve the nitrogen uptake and yield of rapeseed by promoting fine root development
作者：Wang, Chunyun;Wang, Zongkai;Lou, Hongxiang;Wang, Xianling;Shao, Dongli;Tan, Xiaoqiang;Liu, Mengzhen;Wang, Bo;Kuai, Jie;Wang, Jing;Xu, Zhenghua;Zhou, Guangsheng;Zhao, Jie;Wang, Chunyun;Gao, Jianqin;Zhang, Jiefu
关键词：Straw incorporation; Rapeseed; Nitrogen; Root; Yield
-
A rare dominant allele DYSOC1 determines seed coat color and improves seed oil content in Brassica napus
作者：Li, Huaixin;Wu, Mingli;Yin, Yongtai;Xia, Yutian;Cheng, Xin;Chen, Kang;Yan, Shuxiang;Xiong, Yiyi;He, Jianjie;Fan, Shipeng;Ding, Yiran;Zhang, Libin;Jia, Haibo;Li, Maoteng;Chao, Hongbo;Yin, Yongtai;Wang, Xiaodong;Zhang, Libin;Zhang, Chunyu
关键词：
-
Optimized tillage regimes in a rice-oilseed rape rotation system enhance system productivity by delaying post-flowering senescence
作者：Wang, Chunyun;Wang, Zongkai;Lou, Hongxiang;Wang, Xianling;Tan, Xiaoqiang;Shao, Dongli;Liu, Mengzhen;Wang, Bo;Kuai, Jie;Wang, Jing;Xu, Zhenghua;Zhou, Guangsheng;Zhao, Jie;Wang, Chunyun;Gao, Jianqin;Zhang, Jiefu
关键词：Moderate deep tillage; Oilseed rape; Rice; Root; Yield
-
Integrating QTL mapping and GWAS to decipher the genetic mechanisms behind the calcium contents of Brassica napus shoots
作者：Xiang, Yanan;Wang, Xiaodong;Zhang, Jiefu;Xiang, Yanan;Chen, Feng;Shi, Rui;Yang, Tinghai;Zhang, Wei;Zhou, Xiaoying;Wang, Chunyun;Sun, Chengming;Fu, Sanxiong;Wang, Xiaodong;Zhang, Jiefu;Shen, Yue
关键词：
Brassica napus shoots; calcium concentration; QTL mapping; GWAS; candidate genes
