A metabolic enzyme-photosynthetic machinery involved in the co-metabolism of enrofloxacin and ciprofloxacin by Chlorella pyrenoidosa
文献类型： 外文期刊
作者： Wu, Hengyu 1 ; Xiong, Qian 1 ; Tian, Fei 1 ; Wang, Yichun 2 ; Chen, Haigang 1 ; Xie, Fengqi 2 ; Ma, Jiaru 2 ; Tang, Qinglin 2 ; Chen, Yanfen 2 ; Sun, Yuehong 2 ; Li, Hao 2 ; Liu, Yousheng 2 ; Ying, Guangguo 2 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangdong Prov Key Lab Fishery Ecol & Environm, Guangdong Prov Observat & Res Stn Ecosyst Pearl Ri, Guangzhou 510300, Peoples R China
2.South China Normal Univ, SCNU Environm Res Inst, Guangdong Prov Key Lab Chem Pollut & Environm Safe, Guangzhou 510006, Peoples R China
3.South China Normal Univ, Sch Environm, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R China
关键词： Fluoroquinolones; Co-metabolism; Microalgae; Transformation pathway; Key functional genes
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）
ISSN： 0304-3894
年卷期： 2025 年 492 卷
页码：
收录情况： SCI
摘要： The removal of fluoroquinolone antibiotics from wastewater continues to pose significant challenges, as conventional treatment methods often prove ineffective against these persistent pollutants. However, microalgalmediated treatment has emerged as a promising alternative, leveraging its unique potential to degrade recalcitrant contaminants. This study investigates the removal of enrofloxacin (EFX) and ciprofloxacin (CFX) by Chlorella pyrenoidosa, integrating transcriptomics, gene network analysis, and co-metabolic pathways to unravel the mechanisms driving pollutant degradation. Among the four co-metabolic substrates evaluated, glucose and glycine were identified as the most effective in enhancing the degradation of EFX and CFX, respectively. Glycine primarily upregulated genes associated with nitrogen metabolism, while glucose stimulated both photosynthesis and nitrogen metabolism pathways. This synergistic co-metabolic interaction promoted the development of an integrated metabolic enzyme-photosynthetic machinery, which enhanced electron transport, energy generation, catalytic enzyme expression, and extracellular polymeric substance (EPS) production, ultimately leading to a significant increase in the degradation rates of EFX and CFX. Mass balance analysis revealed that biotransformation processes, including defluorination, decarboxylation, hydroxylation, and other transformations, were the predominant mechanism for pollutant removal. Fluorine was detected within microalgal cells using transmission electron microscopy coupled with energy dispersive X-ray spectroscopy (TEM-EDS). A total of eight transformation products (TPs) were identified, and their non-toxic effects on three tested organisms suggest environmentally benign outcomes. These findings provide valuable insights into the mechanisms underlying microalgae-mediated degradation of fluoroquinolone antibiotics and highlight the potential of microalgaebased technologies a sustainable solution for mitigating antibiotic pollution in wastewater.
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