Screening and characterization of sex-linked DNA markers in Mozambique tilapia (Oreochromis mossambicus)
文献类型： 外文期刊
作者： Tao, Wenjing 1 ; Zhu, Xi 1 ; Cao, Jianmeng 2 ; Xiao, Hesheng 1 ; Dong, Junjian 2 ; Kocher, Thomas D. 3 ; Lu, Maixin 2 ; Wang, Deshou 1 ;
作者机构： 1.Southwest Univ, Sch Life Sci, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing,Minist Educ, Chongqing 400715, Peoples R China
2.Chinese Acad Fisheries Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr, Guangzhou 510380, Peoples R China
3.Univ Maryland, Dept Biol, College Pk, MD 20742 USA
关键词： Mozambique tilapia; Genome sequencing and re-sequencing; Sex-linked markers; GMT (genetically male tilapia); Cichlid fish
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2022 年 557 卷
页码：
收录情况： SCI
摘要： The Mozambique tilapia (Oreochromis mossambicus) is an important aquaculture fish. It shows sexual dimorphism in growth, with males growing much faster than females. Thus, production of all-male fingerlings of Mozambique tilapia would improve aquaculture efficiency. However, the lack of accurate sex-specific markers has hampered the mono-sex breeding and the study of the molecular mechanisms of sex determination in this species. Recently, we performed genome sequencing and re-sequencing of female and male Mozambique tilapia and identified the sex chromosome and sex determining region. In the present study, two sex-linked SNP markers were identified and validated. Male specific contigs were assembled from the unmapped reads after aligning male re-sequencing data against the published XX genome. Based on these male-specific contigs, three sex-linked DNA markers, Marker-1, Marker-2 and Marker-3, were developed and correctly identified the genetic sex of 16 fish. The universality of these three male-specific markers was then tested in a panel of 144 individuals, which showed a discrepancy of 5.5% (8/144) between genotypic sex and phenotypic sex. In conclusion, we developed three sexlinked markers to identify different sex genotypes in Mozambique tilapia. This study lays a foundation for the establishment of MAS-GMT (marker-assisted selection of genetically male tilapias) technique and sex-controlled breeding of Mozambique tilapia, as well as for the identification of the sex determining gene.
- 相关文献
作者其他论文 更多>>
-
Effect of Pond-Based Rice Floating Bed on the Microbial Community Structure and Quality of Water in Pond of Mandarin Fish Fed Using Artificial Diet
作者：Jiang, Lijin;Yi, Mengmeng;Cao, Jianmeng;Liu, Zhigang;Wang, Zhang;Lu, Maixin;Ke, Xiaoli;Wang, Miao;Jiang, Zhiyong;Wu, Yuli
关键词：pond-based rice floating bed; mandarin fish; artificial diet; water quality; microbial community structure
-
Genomic and biochemical analysis reveals fermented product of a putative novel Romboutsia species involves the glycometabolism of tilapia
作者：Fan, Zijian;Ke, Xiaoli;Jiang, Lijin;Zhang, Ziyue;Yi, Mengmeng;Liu, Zhigang;Cao, Jianmeng;Lu, Maixin;Wang, Miao;Fan, Zijian;Ke, Xiaoli;Jiang, Lijin;Zhang, Ziyue;Yi, Mengmeng;Liu, Zhigang;Cao, Jianmeng;Lu, Maixin;Wang, Miao
关键词：Romboutsia; tilapia; Carbohydrate; Gut microbiota; SCFAs
-
Molecular characterization, expression and functional analysis of TAK1, TAB1 and TAB2 in Nile tilapia (Oreochromis niloticus)
作者：Zheng, Qiuyue;Liu, Zhigang;Sun, Chengfei;Dong, Junjian;Zhang, Hetong;Ke, Xiaoli;Gao, Fengying;Lu, Maixin;Zheng, Qiuyue;Gao, Fengying
关键词：TAK1; TAB; Immune response; Nile tilapia ( Oreochromis niloticus ); Expression profile
-
Disruption of Zar1 leads to arrested oogenesis by regulating polyadenylation via Cpeb1 in tilapia (Oreochromis niloticus)
作者：Yu, Miao;Zhang, Shiyi;Ma, Zhisheng;Wei, Jing;Sun, Lina;Wang, Deshou;Tao, Wenjing;Qiang, Jun;Kocher, Thomas D.
关键词：Zar1; Oogenesis; Polyadenylation
-
TLR21 is involved in the NF-κB and IFN-β pathways in largemouth bass (Micropterus salmoides) and interacts with TRIF but not with the Myd88 adaptor
作者：Gao, Fengying;Dong, Junjian;Li, Jiaxin;Zhu, Zhilin;Zhang, Hetong;Sun, Chengfei;Ye, Xing;Gao, Fengying;Dong, Junjian;Li, Jiaxin;Zhu, Zhilin;Zhang, Hetong;Sun, Chengfei;Ye, Xing;Li, Jiaxin;Zhu, Zhilin
关键词：TLR21; Largemouth bass ( Micropterus salmoides ); Myd88; TRIF; NF-kappa B; IFN-beta
-
New insights into the role of myostatin in fish fertility based on the findings in mstnb-deficient Nile tilapia (Oreochromis niloticus)
作者：Cai, Jing;Wang, Deshou;Zhou, Linyan;Cai, Jing;Wang, Deshou;Zhou, Linyan;Cai, Jing;Wang, Deshou;Zhou, Linyan
关键词：Nile tilapia; Myostatin; Gene mutation; Fertility
-
Hypogonadotropic hypogonadism in male tilapia lacking a functional rln3b gene
作者：Xu, Qinglei;Zhou, Linyan;Xu, Jian;Ye, Maolin;Su, Yun;Feng, Li;Wang, Deshou
关键词：Nile tilapia; rln3b; Male fertility; HPG axis
