Screening and characterization of sex-linked DNA markers in Mozambique tilapia (Oreochromis mossambicus)
文献类型： 外文期刊
作者： Tao, Wenjing 1 ; Zhu, Xi 1 ; Cao, Jianmeng 2 ; Xiao, Hesheng 1 ; Dong, Junjian 2 ; Kocher, Thomas D. 3 ; Lu, Maixin 2 ; Wang, Deshou 1 ;
作者机构： 1.Southwest Univ, Sch Life Sci, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing,Minist Educ, Chongqing 400715, Peoples R China
2.Chinese Acad Fisheries Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr, Guangzhou 510380, Peoples R China
3.Univ Maryland, Dept Biol, College Pk, MD 20742 USA
关键词： Mozambique tilapia; Genome sequencing and re-sequencing; Sex-linked markers; GMT (genetically male tilapia); Cichlid fish
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2022 年 557 卷
页码：
收录情况： SCI
摘要： The Mozambique tilapia (Oreochromis mossambicus) is an important aquaculture fish. It shows sexual dimorphism in growth, with males growing much faster than females. Thus, production of all-male fingerlings of Mozambique tilapia would improve aquaculture efficiency. However, the lack of accurate sex-specific markers has hampered the mono-sex breeding and the study of the molecular mechanisms of sex determination in this species. Recently, we performed genome sequencing and re-sequencing of female and male Mozambique tilapia and identified the sex chromosome and sex determining region. In the present study, two sex-linked SNP markers were identified and validated. Male specific contigs were assembled from the unmapped reads after aligning male re-sequencing data against the published XX genome. Based on these male-specific contigs, three sex-linked DNA markers, Marker-1, Marker-2 and Marker-3, were developed and correctly identified the genetic sex of 16 fish. The universality of these three male-specific markers was then tested in a panel of 144 individuals, which showed a discrepancy of 5.5% (8/144) between genotypic sex and phenotypic sex. In conclusion, we developed three sexlinked markers to identify different sex genotypes in Mozambique tilapia. This study lays a foundation for the establishment of MAS-GMT (marker-assisted selection of genetically male tilapias) technique and sex-controlled breeding of Mozambique tilapia, as well as for the identification of the sex determining gene.
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