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Transcriptional landscape of sweetpotato root tip development at the single-cell level

文献类型： 外文期刊

作者： Zhao, Nan 1 ; Ding, Xiawei 1 ; Tian, Caihuan 4 ; Wang, Shixin 1 ; Xie, Shuyan 1 ; Zou, Hongda 1 ; Liu, Hao 1 ; Chen, Jingyi 1 ; Lian Liang, Xue 3 ; Huang, Lifei 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Crop Res Inst, Guangdong Prov Key Lab Crops Genet & Improvement, Guangzhou 510000, Guangdong, Peoples R China

2.South China Agr Univ, Coll Agr, Guangzhou 510000, Guangdong, Peoples R China

3.Zhongkai Univ Agr & Engn, Coll Agr & Biol, Guangzhou 510000, Guangdong, Peoples R China

4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

关键词： Ipomoea batatas (L.) Lam.; scRNA-seq; Root; Transitional cells; Developmental trajectory

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Single-cell transcriptome sequencing (scRNA-seq) is a powerful tool for describing the transcriptome dynamics of plant development but has not yet been utilized to analyze the tissue ontology of sweetpotato. This study established a stable method for isolating single protoplast cells for scRNA-seq to reveal the cell heterogeneity of sweetpotato root tip meristems at the single-cell level. The study analyzed 12,172 single cells and 27,355 genes in the root tips of the sweetpotato variety Guangshu 87, which were distributed into 15 cell clusters. Pseudo-time analysis showed that there were transitional cells in the apical development trajectory of mature cell types from stem cell niches. Furthermore, we identified novel development regulators of sweetpotato tubers via trajectory analysis. The transcription factor IbGATA4 was highly expressed in the adventitious roots during the development of sweetpotato root tips, where it may regulate the development of sweetpotato root tips. In addition, significant differences were observed in the transcriptional profiles of cell types between sweetpotato, Arabidopsis thaliana, and maize. This study mapped the single-cell transcriptome of sweetpotato root tips, laying a foundation for studying the types, functions, differentiation, and development of sweetpotato root tip cells. Highlights center dot This is the first single-cell transcriptional atlas of sweetpotato root apex tissue. center dot Single-cell analysis of stem cell niche initiation showed unique transitional states. center dot Sweetpotato, Arabidopsis, and maize root tip cell types were correlated. center dot GATA4 may be involved in regulating sweetpotato root tip development.

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