Tissue culture and Agrobacterium-mediated genetic transformation of the oil crop sunflower
文献类型： 外文期刊
作者： Chen, Fangyuan 1 ; Zeng, Youling 1 ; Cheng, Quan 1 ; Xiao, Lvting 1 ; Ji, Jieyun 1 ; Hou, Xianfei 3 ; Huang, Qixiu 3 ; Lei, Zhonghua 3 ;
作者机构： 1.Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi, Xinjiang, Peoples R China
2.Yili Normal Univ, Coll Biol Sci & Technol, Key Lab Microbial Resources Protect Dev & Utilizat, Yining, Xinjiang, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Econ Crops, Urumqi, Xinjiang, Peoples R China
期刊名称：PLOS ONE （ 影响因子：2.9； 五年影响因子：3.3 ）
ISSN： 1932-6203
年卷期： 2024 年 19 卷 5 期
页码：
收录情况： SCI
摘要： Sunflower is one of the four major oil crops in the world. 'Zaoaidatou' (ZADT), the main variety of oil sunflower in the northwest of China, has a short growth cycle, high yield, and high resistance to abiotic stress. However, the ability to tolerate adervesity is limited. Therefore, in this study, we used the retention line of backbone parent ZADT as material to establish its tissue culture and genetic transformation system for new variety cultivating to enhance resistance and yields by molecular breeding. The combination of 0.05 mg/L IAA and 2 mg/L KT in MS was more suitable for direct induction of adventitious buds with cotyledon nodes and the addition of 0.9 mg/L IBA to MS was for adventitious rooting. On this basis, an efficient Agrobacterium tumefaciens-mediated genetic transformation system for ZADT was developed by the screening of kanamycin and optimization of transformation conditions. The rate of positive seedlings reached 8.0%, as determined by polymerase chain reaction (PCR), under the condition of 45 mg/L kanamycin, bacterial density of OD600 0.8, infection time of 30 min, and co-cultivation of three days. These efficient regeneration and genetic transformation platforms are very useful for accelerating the molecular breeding process on sunflower.
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