The Agropyron mongolicum bHLH Gene AmbHLH148 Positively Involved in Transgenic Nicotiana benthamiana Adaptive Response to Drought Stress
文献类型： 外文期刊
作者： Zhang, Xuefeng 1 ; Ma, Yanhong 1 ; Fan, Bobo 1 ; Sun, Fengcheng 2 ; Zhai, Yongqing 1 ; Zhao, Yan 3 ; Nie, Lizhen 2 ; Fang, Yongyu 2 ; Yu, Zhuo 1 ; Qi, Bingjie 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Agr Coll, Hohhot 010018, Peoples R China
2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China
3.Inner Mongolia Agr Univ, Coll Grassland Resources & Environm, Hohhot 010018, Peoples R China
关键词： Agropyron mongolicum Keng; bHLH transcription factor; AmbHLH148; drought resistance; antioxidant activity
期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN：
年卷期： 2023 年 13 卷 12 期
页码：
收录情况： SCI
摘要： While bHLH transcription factors have been linked to the regulation of various abiotic stressors, research on drought-related bHLH proteins and their molecular processes in Agropyron mongolicum has remained limited. In this study, a bHLH gene from A. mongolicum, designated as AmbHLH148, was successfully cloned and isolated. AmbHLH148 was exclusively localized within the nucleus. Additionally, qRT-PCR analysis demonstrated a significant upregulation of AmbHLH148 in response to drought stress. When transferred into tobacco (Nicotiana benthamiana), the heterologous expression of AmbHLH148 led to enhanced drought tolerance. Under drought stress conditions, AmbHLH148-OE transgenic tobacco plants exhibited increased activities of antioxidant defense enzymes, such as SOD (superoxide dismutase), POD (peroxidase), and CAT (catalase). These enzymes efficiently mitigated the accumulation of reactive oxygen species (ROS) compared to wild-type plants. Furthermore, AmbHLH148-OE transgenic tobacco showed elevated levels of PRO (proline) and reduced MDA (malondialdehyde) content, contributing to enhanced stability in the plant's cell membrane system during drought stress. In summary, this study underscores that the overexpression of AmbHLH148 in transgenic tobacco acts as a positive regulator under drought stress by enhancing the plant's antioxidant capacity. These findings shed light on the molecular mechanisms involved in bHLH transcription factors' role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in A. mongolicum for enhancing crop drought resistance.
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