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SR45a plays a key role in enhancing cotton resistance to Verticillium dahliae by alternative splicing of immunity genes

文献类型： 外文期刊

作者： Liu, Fujie 1 ; Cai, Sheng 1 ; Dai, Lingjun 1 ; Ai, Nijiang 5 ; Feng, Guoli 5 ; Wang, Ningshan 5 ; Zhang, Wenli 1 ; Liu, Kang 1 ; Zhou, Baoliang 1 ;

作者机构： 1.Nanjing Agr Univ, Collaborat Innovat Ctr Modern Crop Prod Cosponsore, State Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Minist Educ, Cotton Germplasm Enhancement & Applicat Engn Res C, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Institue Crop Germplasm & Biotechnol Jiangsu Prov, Zhongling St 50, Nanjing 210014, Peoples R China

4.Nanjing Forestry Univ, 159 Longpan Rd, Nanjing 210095, Jiangsu, Peoples R China

5.Shihezi Agr Sci Res Inst, Xinjiang Prod & Construct Corps, Shihezi 832000, Xinjiang, Peoples R China

关键词： alternative splicing; plant immunity; SR protein; splicing regulating factor; Verticillium dahliae; cotton

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Alternative splicing (AS) of pre-mRNAs increases the diversity of transcriptome and proteome and plays fundamental roles in plant development and stress responses. However, the prevalent changes in AS events and the regulating mechanisms of plants in response to pathogens remain largely unknown. Here, we show that AS changes are an important mechanism conferring cotton immunity to Verticillium dahliae (Vd). GauSR45a, encoding a serine/arginine-rich RNA binding protein, was upregulated expression and underwent AS in response to Vd infection in Gossypium australe, a wild diploid cotton species highly resistant to Vd. Silencing GauSR45a substantially reduced the splicing ratio of Vd-induced immune-associated genes, including GauBAK1 (BRI1-associated kinase 1) and GauCERK1 (chitin elicitor receptor kinase 1). GauSR45a binds to the GAAGA motif that is commonly found in the pre-mRNA of genes essential for PTI, ETI, and defense. The binding between GauSR45a and the GAAGA motif in the pre-mRNA of BAK1 was enhanced by two splicing factors of GauU2AF35B and GauU1-70 K, thereby facilitating exon splicing; silencing either AtU2AF35B or AtU1-70 K decreased the resistance to Vd in transgenic GauSR45a Arabidopsis. Overexpressing the short splicing variant of BAK1GauBAK1.1 resulted in enhanced Verticillium wilt resistance rather than the long one GauBAK1.2. Vd-induced far more AS events were in G. barbadense (resistant tetraploid cotton) than those in G. hirsutum (susceptible tetraploid cotton) during Vd infection, indicating resistance divergence in immune responses at a genome-wide scale. We provided evidence showing a fundamental mechanism by which GauSR45a enhances cotton resistance to Vd through global regulation of AS of immunity genes.

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