您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Integration of GWAS and RNA-Seq Analysis to Identify SNPs and Candidate Genes Associated with Alkali Stress Tolerance at the Germination Stage in Mung Bean

文献类型： 外文期刊

作者： Xu, Ning 1 ; Chen, Bingru 2 ; Cheng, Yuxin 2 ; Su, Yufei 2 ; Song, Mengyuan 2 ; Guo, Rongqiu 2 ; Wang, Minghai 2 ; Deng, Kunpeng 2 ; Lan, Tianjiao 2 ; Bao, Shuying 2 ; Wang, Guifang 2 ; Guo, Zhongxiao 2 ; Yu, Lihe 1 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Agr, Daqing 163319, Peoples R China

2.Jilin Acad Agr Sci, Inst Crop Germplasm Resources, Gongzhuling 136100, Peoples R China

关键词： mung bean; alkali tolerance; genome-wide association study; RNA-seq; candidate genes; germination stage

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 6 期

页码：

收录情况： SCI

摘要： Soil salt-alkalization seriously impacts crop growth and productivity worldwide. Breeding and applying tolerant varieties is the most economical and effective way to address soil alkalization. However, genetic resources for breeders to improve alkali tolerance are limited in mung bean. Here, a genome-wide association study (GWAS) was performed to detect alkali-tolerant genetic loci and candidate genes in 277 mung bean accessions during germination. Using the relative values of two germination traits, 19 QTLs containing 32 SNPs significantly associated with alkali tolerance on nine chromosomes were identified, and they explained 3.6 to 14.6% of the phenotypic variance. Moreover, 691 candidate genes were mined within the LD intervals containing significant trait-associated SNPs. Transcriptome sequencing of alkali-tolerant accession 132-346 under alkali and control conditions after 24 h of treatment was conducted, and 2565 DEGs were identified. An integrated analysis of the GWAS and DEGs revealed six hub genes involved in alkali tolerance responses. Moreover, the expression of hub genes was further validated by qRT-PCR. These findings improve our understanding of the molecular mechanism of alkali stress tolerance and provide potential resources (SNPs and genes) for the genetic improvement of alkali tolerance in mung bean.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台