文献类型： 外文期刊
作者： Yu, Fan 1 ; Li, Jian-Lin 1 ; Feng, Wen-Rong 1 ; Tang, Yong-Kai 1 ; Su, Sheng-Yan 1 ; Xu, Pao 1 ; Zhong, Huan 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China
2.Hunan Agr Univ, Hunan Res Ctr Engn Technol Utilizat Distinct Aqua, Changsha 410128, Peoples R China
关键词： Gynogenesis; Gene co-expression network; Purifying effect; Allogynogenetic biological effect; Genomic stability
期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.727； 五年影响因子：3.782 ）
ISSN： 1436-2228
年卷期： 2022 年 24 卷 2 期
页码：
收录情况： SCI
摘要： Heat shock procedure is crucial for gynogenetic manipulation leading to diploidization of the maternal genomes; however, the underlying molecular mechanism especially the transcriptomic changes during this procedure has still not been unveiled yet. Here, the artificial gynogenesis of zebrafish (Danio rerio) using inactivated sperm from rare minnow (Gobiocypris rarus) was conducted. We found that artificial gynogenetic manipulation, including pseudo-fertilization and heat shock, decreased hatching rates, whereas heat shock treatment alone had medium hatching rates. The first cleavage changed the expression of genes associated with RNA transcription and protein synthesis. A co-expression network regulated by hub genes GIT1, Sepsecs, and FLNB was significantly correlated with heat shock procedure. The cyclin family and cyclin-dependent kinase-related genes were lowly expressed in embryos from gynogenetic zebrafish, and genes involved in controlling the cell cycle and genomic stability were significantly altered by the gynogenetic treatment. Our results show the effects of artificial gynogenesis on embryos and describe changes in gene expression that suggest drastic changes take place in cell division by heat shock procedure. These findings will contribute to an understanding of the molecular basis for germplasm improving, including the purifying effect and allogynogenetic biological effect by gynogenesis.
- 相关文献
作者其他论文 更多>>
-
Aquaculture in Zambia: The Current Status, Challenges, Opportunities and Adaptable Lessons Learnt from China
作者：Zhang, Lin;Hua, Fenglei;Xu, Pao;Zhang, Lin;Hua, Fenglei;Xu, Pao;Maulu, Sahya;Maulu, Sahya;Chama, Majory K. H.
关键词：Zambia; aquaculture; challenges; adaptable lessons
-
Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water
作者：Mang, Qi;Xu, Gangchun;Xu, Pao;Mang, Qi;Gao, Jun;Li, Quanjie;Sun, Yi;Xu, Gangchun;Xu, Pao
关键词：probiotics; ammonia; nitrite; nitrogen cycling; nitrification; denitrification
-
Integrative analysis of miRNA-mRNA expression in the brain during high temperature-induced masculinization of female Nile tilapia ( Oreochromis niloticus )
作者：Jiang, Bingjie;Lu, Siqi;Dong, Yalun;Xu, Pao;Qiang, Jun;Tao, Yifan;Li, Yan;Xu, Pao;Qiang, Jun;Badran, M. F.;Qiang, Jun;Tao, Yifan
关键词：Oreochromis niloticus; Brain tissues; High temperature -induced; Sex reversal; miRNA-mRNA expression
-
A High-Fat-Diet-Induced Microbiota Imbalance Correlates with Oxidative Stress and the Inflammatory Response in the Gut of Freshwater Drum (Aplodinotus grunniens)
作者：Xue, Miaomiao;Xu, Pao;Wen, Haibo;Chen, Jianxiang;Wang, Qingyong;He, Jiyan;He, Changchang;Kong, Changxin;Li, Xiaowei;Li, Hongxia;Song, Changyou;Xu, Pao;Wen, Haibo;Chen, Jianxiang;Li, Hongxia;Song, Changyou
关键词：high-fat diet; gut microbiota; physiological homeostasis; Aplodinotus grunniens
-
Comparative transcriptomics and metabolomics reveal specific biochemical responses in cultured largemouth bass ( Micropterus salmoides ) juveniles with sexual dimorphism
作者：Li, Yan;Wang, Qingchun;Qiang, Jun;Xu, Pao;Li, Yan;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Dong, Yalun;Jiang, Bingjie;Qiang, Jun;Xu, Pao
关键词：Largemouth bass; Sexual dependence; Lipid droplets; Differentially expressed genes; Differential metabolites
-
H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics
作者：He, Qinghong;Tang, Yongkai;Feng, Wenrong;Chen, Xue;Xu, Yuanfeng;Li, Jianlin;Xu, Pao;Tang, Yongkai;Zhou, Jun
关键词：Eriocheir sinensis; oxidative stress; antioxidation; gene expression; H2O2
-
A study on the structure-functionality relationship of Solenaia oleivora protein under high-intensity ultrasonication processing
作者：Chen, Wanwen;Jin, Wu;Ma, Xueyan;Wen, Haibo;Xu, Gangchun;Xu, Pao;Chen, Wanwen;Jin, Wu;Ma, Xueyan;Wen, Haibo;Xu, Gangchun;Xu, Pao;Chen, Wanwen;Jin, Wu;Ma, Xueyan;Wen, Haibo;Xu, Gangchun;Xu, Pao;Cheng, Hao;Cheng, Hao;Li, Yanping
关键词：Shellfish; Protein; Ultrasonication; Structure; functional property; digestibility
